Nuclear & Cytoplasmic Staining
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| What are the two types of nucleic acids? | DNA and RNA
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| This type of nucleic acid found in the nucleus has a 5 carbon sugar called deoxyribose and its major constituent is nuclear chromatin? | DNA
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| This type of nucleic acid found in the nucleus and in the ribosomes contains a 5 carbon sugar called ribose? | RNA
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| The sugars in DNA and RNA differ by a single or a double hydroxl group? | Single
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| The stain demonstates the presence of DNA? | Feulgen Reaction
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| This stain differentiates between RNA and DNA and is also used as a connective tissue stain to identify plasma cells and immunoblasts? | Methyl Green Pyronin Y
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| This stain differentiates cells present in hematopoietic tissue? | Giemsa
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| This stain is considered the most specific to demonstate amyloid? | Alkaline Congo Red
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| This stain is a rapid screening method to demonstate amyloid? | Crystal Violet
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| This stain is a fluorescent stain to demonstrate amyloid? | Thioflavin T Fluorescent Method
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| For the fixation of this stain you can use ANYTHING except BOUIN'S? | Feulgen Method
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| This substance comprises 1-2% carbohydtrate (chrondriotin, heparin and dermatan) and acid mucopolysaccharides. | Amyloid
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| A disease characterized by an amorphorous, eosinophilic, extracelular deposits? | Amyloidosis
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| This disease gradually replaces cellular elements of vital organs and causes progressive loss of function and eventual cell death? | Amyloidosis
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| This type of amyloidosis occurs spontaneously in the absence of any predisposing disease? | Primary Amyloidosis
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| This type of amyloidosis is assocoated with predisposing diseases such as chronic inflammatory diseases? | Secondary Amyloidosis
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| Chronic inflammatory disease such as rheumatoid arthritis and ankylosing spondylitis causing infections such as tuberculosis and osteomyelitis are associated with which type of amyloidosis? | Secondary Amyloidosis
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| This type of amyloidosis is assocoated with disease of the immunological system? | Myeloma-Associated Amyloid
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| This type of amyloidosis is assocoated with many tumors, especially APUD (amine precursor uptake and decarboxylation) | Tumor Associated Amyoid
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| The organs most effected with this type of amyloidosis are muscle, heart, skin and tongue? | Primary Amyloidosis
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| The organs most effected with this type of amyloidosis are kidneys, liver, spleen and adrenal gland? | Secondary Amyloidosis
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| What color does DNA stain in the Feulgen stain for DNA? | DNA stains magenta/reddish purple
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| In the Methyl Green Pyronin Y stain, what color do DNA and RNA stain? | DNA green or blue/green. RNA red.
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| What color do nuclei and bacteria stain in the giemsa polychromatic stain? | Blue
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| For Congo Red, what color does Amyloid stain? | Amyloid stains deep pink to red with a light micropsope and bright green apple with a polarizing microscope.
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| What color does amyloid stain in the Crystal Violet stain? | Purplish/violet
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| What color does amyloid stain in the Thioflavin T Fluorescent stain? | Fluorescent yellow to yellow/green.
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| The principle of this stain is that it is a combination of acid hydrolysis and aldehyde staining? | Feulgen Reaction for DNA
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| A principle of the stain is differential staining is due to the differing degrees of polymerization between DNA and RNA? | Methyl Green Pyronin Y
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| What is the T control for Methyl Green Pyronin Y? | Section containing many plasma cells
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| What is a control for amyloid stains? | Any tissue containing amyloid
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| What are three stains that stain for amyloid? | Congo Red. Crystal Violet and Thioflavin T
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| What is the contriol for Giemsa? | Spleen
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| The principle for this stain is a dye attaches to amyloid and requires no differentiation? | Thioflavin T
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| What do the three amyloid stains cut at? | Congo Red 8-10. Cryastal Violet 10-12. Thioflavin T 6-10
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| What fixatives are used for the three amyloid stains? | Alcohol or Carnoy is preferred for Congo Red and 10% NBF for Crystal Violet and Thioflavin T.
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| Pretreatment with Alkali aids in the relase of native internal hydrogen bonds between adjacent protein chains creating more sites for the dye to bind is a principle for what stain? | Congo Red
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| A principle of this stain is the addition of HCl in the stain prevents the cytoplasmic component from overstaining? | Crystal Violet
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| The principle of this stain is that a basophilic dye methylene blue is combined with acidic dyes eosin, azure A and azure B to create a neutral dye that demonstrates a wide variety of colors when used to stain hematopoietic cell nuclei or platelets? | Giemas for hematopoietic tissue
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