Chemistry Shecimen & Controls Wooksheet
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| What four things does an accurate chemical analysis depend on? | Proper collection / preservation / Processing / Method of analysis (Machine)
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| What is the ideal time for lab measurements to be performed? | 1 hr after drawing blood - contents change after that
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| What is the three reasons that specimens are preserved? | 1. So that substance to be measured is not altered; 2. Samples should be retained long enough to repeat if necessary (Reflex) 3. Specimen collected in a timed sequence should be stored until they can be checked
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| What two things must you remember about controls? | Verify unopened & opened Experation dates; Control results published by lot number
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| Where do controls come from? | Large pools of serum or plasma
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| What sample is most often used for chemical analysis? | Serum (Red, Gold, Yellow Tubes)
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| Define fasting | No food or drink for 12 hours.
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| What is the difference between serum and plasma? | Plasma circulating blood contains fibrinogen clotting agents / Serum liquid portion w/ fibrinogen and clotting factures removed.
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| Which portion of blood sits on top of the clot? | Serum
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| Which part of the blood contains fibrinogen? | Plasma
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| What does FPG stand for? | Fasting Plasma Glucose
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| What is glycolysis? | Blood that as set too long and used up glucose
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| What tests require fasting? | Triglycerides (Fats), Glucose, Lipid Panel
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| Why is fasting beneficial? | Provides metabolic baseline / Eliminates lipemia (Lipids in the blood)
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| What is the actual name of the silicone barrier that separates serum & plasma? | Thyrotrophic gel
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| What does thyrotrophic gel do? | Prevent shift of substances /Prevents glycolysis
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| What vacutainer is used to collect serum and why? | Gold or red / Contains no anticoagulant
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| What is step one to processing a serum (Gold) tube? | Invert 5 x; do not centrifuge, yet
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| What is step two to processing a serum (Gold)tube? | Let sit no less then 30 mins - no more the 60 mins to form clot
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| What is step three to processing a serum (Gold)tube? | Centerfuge at least 15 mins within 1 hr of drawing
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| What is step four to processing a serum (Gold)tube? | Transfer to a labeled plastic bottle and write serum on it
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| What is step five to processing a serum (Gold)tube? | Refrigerate or freeze
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| Define hemolyzed serum | Is red instead of yellow
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| Define lipemic | Serum is milking white from fat (result of drawing too soon after eating.)
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| Define accuracy | True or actual valve (Controls check accuracy)
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| Define precision | Repeatablity - (Ablility to get the same answer twice.)
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| Define normal value | Average result of healthy individuals / (+/- 2 SD - standard deviation 95%)
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| Define Batch | Specimen (samples) analyzed at the same time
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| Define standard (also called calibrator) | Used to set machine values
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| Define control | Man made specimens used to verify machine accurate function. Must be done before pt.
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| Define proficiency survey | specimens sent to your lab to verify lab can get same readings
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| What four things does the control check for? | Reagents, machine function, operator technique, environment (Temp & humidity)
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