Biotech I Terms for Buffer Preparation
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Acid | any substance that releases hydrogen ions when dissolved in water
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| Aliquot | to divide a solution into small, equal individual containers
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| Base | any substance that releases hydroxide ions when dissolved in water, any substance that can accept hydrogen ions
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| Concentration | an amount per volume; a fraction
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| Formula Weight (molecular wt) | the weight in grams of 1 mole of a given compound. The FW/MW is calculated by adding atomic weights of the atoms that compose the compound
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| Hydrophobic | "hating" water, insoluble in water such as fats and oils
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| Hydrophilic | "loving" water, readily dissolves in water such as salt or sugar
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| Lyophilization | freeze drying, a preservation method, rapid freezing and then drying under vacuum to remove the water
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| Molarity | an expression of the concentration of a solute in a soln. that is the number of moles of a solute dissolved in 1 L of total solution
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| Mole | a mole of any element contains 6.02x10 23 atoms. Moles of elements weigh different amounts but they all contain 6.02x10 23 molecules of that compound
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| QS | Latin abbreviation meaning quantum sufficit; bring to volume
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| Sensitivity | the smallest weight which can be weighed on a scale
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| Capacity | the heaviest weight which can be weighed on a scale
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| Range | the total span a balance can weigh from lightest to heaviest
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| Calibration | Adjustments made to an outside standard
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| Verification | confirming daily that the equipment is working properly, use of calibrated weights or pH buffer checks
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| Percision | the ability to get the same result over and over
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| Accuracy | getting the result you expect each time
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| Tolerance | the amount of error you are willing to accept; tighter the tolerance the less error you will accept
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| Equipment log book | where all verification and calibration records are stored
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| Lab notebooks | legal documents, composition book, tracked and accounted for
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