CH19 - Analyzing and Engineering Genes
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| What is the function of REVERSE TRANSCRIPTASE? | The function of reverse transcriptase is similar to the reverse of transcription; it makes a single strand of complementary DNA (cDNA) from mRNA. It makes complementary DNA (cDNA) from mRNA.
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| How can large quantities of protein be produced from a bacterial colony containing the gene of interest? | Put a cDNA encoding the protein into a plasmid with a bacterial promoter sequence. Once a cDNA is put into a plasmid with a bacterial promoter, the bacteria will transcribe and translate the gene.
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| What are "sticky ends"? | They are the overhanging ends on DNA that are generated by restriction enzymes, which can base pair with any DNA molecules that contain complementary sticky ends
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| What is the polymerase chain reaction (PCR)? | A method to amplify a fragment of DNA. PCR is an in vitro DNA synthesis reaction that produces many copies of a single DNA fragment.
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| What information can not be obtained from the sequence of a gene? | Cellular location of the protein.
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| What is the usefulness of DNA SEQUENCE COMPARISONS? | DNA sequence comparisons are a powerful tool by which to estimate how closely related different species are along the evolutionary timeline.
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| During which step in the PCR cycle are nucleotides used? | Extension. Nucleotides are used to synthesize the complementary strand to the DNA template during the extension step.
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| During which step in the PCR cycle do primers form bonds with a single-stranded template? | Annealing
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