Technique
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Bacterial proteins that cleave DNA at specific sequences | Restriction enzymes
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| A palindrome and a restriction site | GAATTC
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| The human ... gene is removed from human DNA and subcloned into a .... The bacteria then produce ... which can be used to treat type I and II diabetes | Insulin, Bacterial plasmid, Human insulin
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| DNA is negatively charged | True
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| DNA is separated on gel electrophoreis based on | Molecular weight
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| Technique used to identify DNA | Southern Blot
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| Technique used to identify RNA | Northern Blot
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| Technique used to identify Protein | Western Blot
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| A single-stranded piece of DNA or RNA that is used to visualize complementary sequences within DNA | Probe
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| Utilizes dideoxy nucleotides to sequence segments of DNA | DNA sequencing
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| A technique used to amplify DNA | Polymerase Chain REaction (PCR)
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| Derived from teh bacteria Thermus Aquaticus commony found in hot springs. Use in PCR | Taq polymerase
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| A technique used by researchers to knockout a particular gene of interest | Gene Knockout
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| A technique used to identify DNA chromosomal abnormalities. For example, trisomies, monosomies, and various other abnormalities that can be detected by karyotypes. | Karyotype
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| A technique used to create an exact copy of oneself. | Cloning
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| Inhibitor RNAs | RNAi
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| Used to inhibit protein expression by inducing the degradation of mRNA | RNAi
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| The enzyme responsible for cleavage or RNAi into siRNA | Dicer
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| The enzyme responsible for cleavning the endogenous mRNA | RNA Induced Silencing Complex (RISC)
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| Macular Degeneration, Hypercholesterolemia, RSV, Hepatitis C | Disease currently being investigated for RNAi treatment
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Created by:
mhaynes
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