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Cell Bio/Histo/Path -3

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Inflammation: Classic signs   -Rubor (redness) -Dolor (pain) -Calor (heat) -Tumor (Swelling) -Functio lassa (Loss of function)  
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Characteristics of Inflammation: Fluid exudation   1. Increased vascular permeability 2. Vasodilation 3. Endothelial injury  
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Characteristics of Inflammation: Leukocyte activation   1. Emigration 2. Chemotaxis 3. Phagocytosis and killing  
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Characteristics of Inflammation: Fibrosis   1. Fibroblast emigration and proliferation 2. Deposition of extracellular material  
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Characteristics of Acute Inflammation   Mediated by: 1. Neutrophils 2. Eosinophils 3. Antibodies  
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Characteristics of Chronic Inflammation   Mediated by mononuclear cells: -Characterized by persistant destruction and repair -Granulomas: nodular collections of macrophages and giant cells  
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Characteristics of Inflammation: Resolution   1. Restoration of normal structure 2. Granulation tissue 3. Abscess 4. Fistula 5. Scarring  
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Granulation tissue: histologic characteristics   -highly vascularized -fibrotic  
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Abscess: histologic characteristics   fibrosis surrounding pus  
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Fistula: characteristics   abnormal communication  
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Scarring: histologic characteristics   Collagen deposition resulting in altered structure and function  
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Steps in leukocyte emigration   1. Rolling 2. Tight binding 3. Diapedisis 4. Migration  
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Leukocyte emigration step characteristics: Rolling   Binding between: -E-selectin on vascular endothelium -Sialyl-LewisX on the leukocyte  
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Leukocyte emigration step characteristics: Tight binding   binding between: -ICAM-1 on vascular endothelium -LFA-1 on leukocyte  
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Leukocyte emigration step characteristics: Diapedesis   leukocyte travels between endothelial cells and exits blood vessel  
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Leukocyte emigration step characteristics: Migration   Leukocyte travels through interstitium to the site of injury or infection guided by chemotactic signals  
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Chemotactic signals   1. Bacterial products 2. Complement 3. Chemokines  
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Free radical injury: Initiated by what?   1. Radiation exposure 2. Metabolism of drugs (phase I) 3. Redox reactions 4. Nitric oxide 5. Transition metals 6. Leukocyte oxidative burst  
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Free radical injury: Mechanism   -Membrane lipid peroxidation -Protein modification -DNA breakage  
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Free radical injury: Stopped by what?   -Spontaneous decay -Antioxidants --Vitamin E --Vitamin A -Enzymes --Catalase --Superoxide dismutase --Glutathione peroxidase  
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Major cause of injury after thrombolytic therapy   Free-radical production induced by reperfusion after anoxia  
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Hyperplasia: definition   reversible increase in number of cells  
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Metaplasia: definition   Reversible substitution of one cell type for another.  
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Metaplasia: When found?   Often secondary to irritation and/or environmental exposure (eg squamous metaplasia in trachea and bronchi of smokers)  
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Dysplasia: definition   Reversible abnormal growth with loss of cellular orientation, shape and size in comparison to normal tissue maturation  
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Dysplasia: When found?   In paraneoplastic syndromes  
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Anaplasia: definition   abnormal cells lacking differentiation, like primitive cells of same tissue.  
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Anaplasia: When found?   Undifferentiated malignancies  
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Neoplasia: Definition   A clonal proliferation of cells that is uncontrolled and excessive  
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Difference between hyperplasia and dysplasia   Can occur together 1. hyperplasia - increase in number 2. dysplasia - abnormal proliferation of cells with loss of size, shape, and orientation  
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Appearance of cancerous cells   -High nuclear/cytoplasmic ratio -Clumped chromatin  
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How does carcinoma invade a basement membrane?   -Collagenases -Hydrolases  
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Seed and soil theory of metastasis   Seed: Tumor embolus Soil: Target organ  
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Tumor grade: definition   Degree of cellular differentiation based on histologic appearance of tumor  
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Tumor stage: definition   Degree of localization/spread based on site and size of primary lesion, spread to regional lymph nodes, and presence of metastases  
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Tumor grade vs stage: Quick characterization   grade: character of tumor itself stage: spread of tumor in a specific patient (Stage=Spread)  
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Tumor grade vs stage: Which has more prognostic value?   Stage  
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TNM staging system   Tumor size Node involvement Metastases  
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Benign tumors of cell type: Blood cells   Does not exist. The malignant blood cells are automatically metastasizing.  
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Benign tumors of cell type: Blood vessels   Hemangioma  
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Benign tumors of cell type: Smooth muscle   Leiomyoma  
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Benign tumors of cell type: Skeletal muscle   Rhabdomyoma  
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Benign tumors of cell type: Bone   Osteoma  
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Benign tumors of cell type: Fat   Lipoma  
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Benign tumors of cell type: >1 cell type   Mature teratoma (women)  
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Benign tumors of cell type: Epithelium   -Adenoma -Papilloma  
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Malignant tumor of cell type: Epithelium   -Adenocarcinoma -Papillary carcinoma  
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Malignant tumor of cell type: Blood cells   -Leukemia -Lymphoma  
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Malignant tumor of cell type: Blood vessels   Angiosarcoma  
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Malignant tumor of cell type: Smooth muscle   Leiomyosarcoma  
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Malignant tumor of cell type: Skeletal muscle   Rhabdomyosarcoma  
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Malignant tumor of cell type: Bone   Osteosarcoma  
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Malignant tumor of cell type: Fat   Liposarcoma  
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Malignant tumor of cell type: >1 cell type   Immature teratoma, Mature teratoma (men only)  
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