Microbiology Test #2 Review Dr. Bharathi
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| What is an organism that produces superoxide dimutase and catalase? | Aerobe
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| What is not a disadvantage for a direct microscope count? | Sample volume of an unknown.
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| What are the disadvantages of direct microscope count? | Motile bacteria are difficult to count, dead cells are likly to be counted, high concentrations of cells are requred to be countable.
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| What is the advantage of direct microscope count? | No incubation time is required.
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| What do hydrothermal vents on the ocean floor useas a carbon source? | CO2
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| What is in nutrient agar? | peptone & beef extract
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| What do producers in the vents use? | sulfides
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| What is the direct method to count microbial growth? | metabolic activity
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| What is the best pH for most bacteria? | 7
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| What is the best temperature for optimum growth of mesophiles? | 25
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| What is the best temperature for optimum growth of psychrophiles? | 15
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| What is the best temperature for optimum growth of psychotrophs? | 20-30
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| What catalyzes 2H202---into 2H2O + O2? | Catalase
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| What is an organic growth factor? | NAD+
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| What is not used to determine the metabolic growth factor? | Turbidity
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| What is an organism that uses oxygen or grows without oxygen? | facultative anaerobe
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| What is not a disadvantage to determine viable cells? | viable plate count
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| How do salts and sugars preserve food? | creates a hypertonic environment
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| What makes H202 + 2H+ ----into 2H20? | peroxidase enzyme
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| What is a common intermediate between catabolic and anabolic pathways? | ATP
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| When are gram positive bacteria most susceptible to penicillin? | Log phase
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| What organisms are capable of respiration? | anaerobic
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| What is generated by the flow of protons across the cell membrane? | ATP
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| What neutralizes acids in the medium? | buffers
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| What measures microbial growth? | metaboic activity & glocose consumption
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| Inwhat mediums do gram positive organisms grow such as staphylococcus aureus colonies? | selective & differential
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| How do gram positive organisms grown in selective & differential mediums look? | yellow halo surrounds
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| What is a step in glycolysis? | oxidation
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| What do aeobic and anaerobic respiration have in common? | final receptor of electrons are different in both
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| What is the oxidation of glucose with organic molecules serving as electron receptors? | fermintation
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| What is not necessary for respiration? | oxygen
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| What is an example of cyanobacteria? | photoautotroph
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| What uses CO2 for carbon and H2 for energy? | chemoautotroph
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| What is involved in aerobic respiration and oxidized in the Krebs Cycle? | pyruvic acid
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| What happens to a bacterial culture grown in glucose? | peptide medium causes the pH to increase most likely using peptides
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| What does phosphoeno pyruvatie into pyruvic acid generate? | ATP
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| What is happening when ATP general when protons are moved across a membrane? | chemiosmotic mechanism
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| What does photophosphrylation require? | CO2
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| What is not an enzyme? | Coenzyme A
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| What is not an end product of fermentation? | Pyruvic acid
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| What has bacteria chlorophylls and uses alcohols for carbon? | photoheterotrophs
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| Fermentation is defined as what? | Metabolic process
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| Fermentatin releases what? | energy from sugar
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| Fermentation does not require what? | oxygen
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| How does fermentation occur? | anaerobically
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| What is the oxidation of intrmediate metabolic compounds? | substrate level phosphorylation
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| What is metabolized in the Krebs Cycle or citric acid cycle? | fattyacids
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| What is the energy released as carrier molecules and are oxidized and used to generte ATP? | oxidative phoshorylation
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| What does not produce 3 ATPs? | pentose hophate pathway
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| What uses glycose for carbon and energy? | chemoheterotroph
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| How does a strictly fermentative bacteria produce energy? | glycolyis only
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| What is not an energy source for a photautotroph? | CO2
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