UND 363 Bile stain (Hall)
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| purpose for Hall Bile stain | demo presence of bilirubin in tissue and distinguish bile pigments (as a result of red cell breakdown)
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| principle for Hall Bile stain | oxidation of bilirubin to biliverdin in acid medium (turns green). oxidation done by ferrich chloride in trichloroacetic acid medium (AKA FOUCHETS REAGENT)
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| fix for Hall Bile stain | NBF
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| tech | 4-5 and FS
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| qc | tissue with bile (eg obstructed bile duct)
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| reagents for Hall Bile stain | fouchets (ferric chloride 10% and trichloracetic acid) - oxidation, acid fuchsin/picric acid (van gieson) - counterstain
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| results | bile/bilirubin - green (emerald - olive), background - yellow
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