Bacteria Bd Cultures
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| Bacteria in the blood ? | * Bacteremia
🗑
|
||||
| systemic inflammatory response syndrome, SIRS, has to have 2 out of what 4 conditions ? | * Temp >38oC
--- Heart rate >90 beats/min
--- Respirations >20 breaths/min
--- WBC >12,000/cmm or with >10% bands (left shift)
🗑
|
||||
| SIRS due to an infection ? | * Sepsis
🗑
|
||||
| Sepsis associated with organ dysfunction, hypoperfusion, or hypotension ? | * Severe Sepsis
🗑
|
||||
| Persistent hypotension and perfusion abnormalities despite adequate fluid resuscitation ? | * Septic Shock
🗑
|
||||
| Altered organ function in acutely ill patient such that homeostasis cannot be maintained without intervention ? | * Multiple Organ Dysfunction Syndrome (MODS)
🗑
|
||||
| What is a left "shift" ? | * caused by the release of Neutrophils in response to bacteria
🗑
|
||||
| Process of someone having Organ Failure ? | * Bacterial infection --> Bacteremia --> Host inflammatory response --> Increased Inflammation & Coagulation/Decreased Fibrinolysis --> Changes in endothelium, microvasculature, BP --> Shock & Organ failure
🗑
|
||||
| Homeostasis and Sepsis ? | * The pathophysiology of sepsis includes the activation of inflammation, the activation of coagulation, and impairment of fibrinolysis......* this creates an imbalance in procoag and anticoag activity
🗑
|
||||
| Septic Shock and mortality ? | * Once septic shock has hit, mortality is very high
🗑
|
||||
| Pathophysiology of Bacteremia ? | * Primary focus of infection → lymphatics → vascular system
🗑
|
||||
| Some sources of Bacteremia ? | * Urinary tract --- Respiratory tract --- Intra-abdominal sites --- Abscesses and surgical wounds --- Intravascular devices ...... * Usu from Stap. Epi form our normal skin flora
🗑
|
||||
| Clinical Patterns of Bacteremia ? | * Transient - very common and cleared quickly, usu from defecating or brushing teeth ....... * Intermittent - subacute endocarditis or some abscesses .......* Continuous - acute endocarditis and some deep seated infections
🗑
|
||||
| Some things that predispose you to sepsis or septic shock ? | * Young/Old
- Alteration in host defenses (spleen loss)
- Severe underlying illnesses: DM/Cancer
- Surgery
- foreign bodies
- RF requiring dialysis
- Obstructive processes
- Tmt w/ cytotoxic or antimetabolites
- Loss of skin barrier
🗑
|
||||
| Common Septic agents today ? | * Staphylococcus aureus
- Escherichia coli
- Coag neg Staphylococcus
- Klebsiella pneumoniae
- Enterococcus sp.
- Candida albicans
- Pseudomonas aeruginosa
- Streptococcus pneumoniae
- Streptococcus viridans grp
- Enterobacter cloacae
🗑
|
||||
| Agents it was back in the day ? | * E. Coli was 1st
🗑
|
||||
| Blood Culture Collection ? | * Collect after decontamination --- *Draw When Fever Peaks --- *Number of cultures:
Acute sepsis: 2-3 blood cultures 30 min apart
....Subacute sepsis: 3 or more cultures over 1-2 days
🗑
|
||||
| Principles of Lab Detection ? | * need atleast 10ml of Bd..... * 1ml Bd to 10 ml broth ..... * Anticoagulant use:
Sodium polyanetholsulfonate (SPS) .....* remove microbes
🗑
|
||||
| Blood Culture Systems: Traditional bottles and septi-check ? | * one is the old way, and the other attaches a medium to the top of the bottle and invert occasionally to see if anything grows
🗑
|
||||
| Blood Culture Systems: Lysis centrifugation and Automated Bactec 9000 ? | * Lysis: lysis RBCs to leave organism, and is good for YEASTS .... * Bactec = Detects growth by monitoring CO2 production, bye using different media bottles to grow different bacteria types
🗑
|
||||
| Detects growth by monitoring CO2 production ? | * Clorometric CO2 monitoring...newest way
🗑
|
||||
| Advantages to continuous blood monitoring systems ? | * Earlier detection time and less lab work time
🗑
|
||||
| Culture Interpretation ? | * Identify the Organism (contaminant or pathogen) ...... * Patterns of Positivity (repeat to rule out) .... *
🗑
|
||||
| False-positive rates of organisms that frequently represent contamination ? | * Coag neg Staph --- Corynebacterium spp --- Bacillus spp --- Propionibacterium acnes --- Viridans group Strep .............. * All due to contamination issues
🗑
|
||||
| Bd culture parameters ? | * best is one stick with multiple bottles, but hardly anyone does this
🗑
|
||||
| Who needs a Bd Culture ? | * Need 1 Major Criteria - suspected endocarditis, >39.4 temp (103), Vasc Cath. ...... * Or 2 Minor = Chills, Vomitting ,Fever 38.3-39.3C, Over 65yo, systolic BP <90, Cr > 2, > 18,000 WBC
🗑
|
||||
| Empiric Treatment ? | * start within 1 hr of suspicion, culture and then adjust.... * Cover broad spectrum for both G+ and - ....... * Vancomycin + Gram negative coverage
🗑
|
||||
| Factors leading to poor prognosis ? | * elderly -- see septic shock -- no febrile response -- underlying issues (cancer/DM/HTN) -- Enterococcal/G - etiology
🗑
|
||||
| Bd Culture Message ? | * Up to half of bd cultures are contaminated -- identify the organism can make it easier when see if it is contaminated or not -- repeated bd cultures show same organism -- contamination can be lowered by antiseptic use and well trained personel
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
thamrick800
Popular Medical sets