UND 363 Crystal Violet
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| Purpose of Crystal Violet | demonstrate amyloid (not a specific as congo red)
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| principle of Crystal Violet | *not clearly understood*
polychromatic (ie single dye that stains components different colors)
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| the addition of acid to Crystal Violet will prevent what | overstaining of the cytoplasm
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| fixatives of Crystal Violet | 10% NBF or alcohol
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| thickness and control | 10-12 micron, control must be a known positive for amyloid
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| microscope of Crystal Violet | light
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| name the 2 reagents for crystal violet | crystal violet - stains amyloid
apathy mounting media
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| why is apathy mount media needed | there is potential bleeding of basic aniline dyes into surrounding mouting medium when aqueous media is used
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| what does apathy mount have that prevents bleeding | sugar and salt
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| what can be used instead of apathy and how | synthetic resins can be used but stained sections must be completely air dried and then dipped in xylene before coverslipping
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| typical results of Crystal Violet | amyloid - purplish violet
other tissue - blue
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Created by:
mustangvxd
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