UND 363 Alcian Blue PAS Hematox
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| purpose of A.B. PAS Hematox | differientate between neutral and acidic mucosubstances
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| Principle of A.B. PAS Hematox | acidic mucosubstances stain with alcian blue while neutral mucosubstances stain with PAS
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| Fixatives for A.B. PAS Hematox | 10% NBF or Zenkers
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| Thickness technique | routine tissue at 4-5 microns
kidney at 2-3 microns
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| Control slides for A.B. PAS Hematox | use kidney or mucin control, using either depends on the diagnostic tissue that will be stained (can also use endo/ectocervix)
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| Microscope for A.B. PAS Hematox | Light
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| name the 5 major reagents for A.B. PAS Hematox | acetic acid 3% , alcian blue, periodic acid *0.5%*, schiff, harris hematox
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| what does the acetic acid and alcian blue do in the A.B. PAS Hematox | acetic acid 3% protects the ph, alcian blue will stain the acidic mucosubstances
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| what does the Periodic Acid and schiff reagent do in A.B. PAS Hematox | Periodic acid 0.5% - oxidizes glycols to aldehydes, Schiff reagent will stain the neutral mucosubstances
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| what is the purpose of the harris hematox in A.B. PAS Hematox and what issue may arise and what can fix it. | acts as counterstain, easy to overstain (1 min. per book may be too long) **if overstained use rapid rinse of 0.1HCL/70%ethanol mixture
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| what are the expected results of A.B. PAS Hematox | EXCUSIVELY acid mucosubstances - blue,
neutral poly saccharides - magenta
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