Microbiology Specimen Collection, Transport and Processing
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| Body Fluids (peritoneal, synovial, pleural, etc) | Sterile, screw-cap container or anaerobic transporter | Disinfect aspiration site with alcohol swab and Betadine | Needle aspiration are preferable to swab collections | Transport immediately to laboratory
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| Eye | Aerobic transport swab | Pull lower lid down while gently collecting exudate slong rim | Trahsport swabay be stored up to 24 hrs at room temp
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| Stool | Clean, leak proof container | Outpatients: At minimum, three specimens are collected every other day | Transport to lab within 24 hr at 4*C | Plate within 72 hrs if storing at 4*C
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| Rectal swab | Swab placed directly in enteric transport medium | Insert swab approx 1 in past anal sphincter | Store at 4*C, transport within 24 hr to lab and place within 72 hr
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| Gonorrhea culture | Jembec transport system or transport device with Stuart's or Amie's medium | Wipe away exudate before obtaining culutre specimen, obtain culture specimen with swab | Do not refrigerate | Transport to lab within 2 hrs
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| Chlamydia culture | Specialized transport medium containing antibiotics | Urogenital swab preferred; necessary to obtain epithelial cells, not exudate | Transport immediately on ice to lab | Store up to 24 hr at 4*C; inoculate within 15 min of collection if swab is not on ice
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| Skin scraping for fungal culture | Clean, screw-top tube | Wipe skin with alcohol prep pad | Scrape skin at leading edge of lesion | Can be held indefinitely at room temp but best to process within 72 hrs of collection
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| Sputum | Sterile, screw-cap container | Patient should rinse or gargle with mouthwash before collection | Have patient collect from deep cough; do not collect saliva | Store at 4*C and plate within 24 hr
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| Throat | Transport swab | Have patient sitting with head tilted back | Swab pharynx and tonsils,not mouth, tongue or teeth | Transport and plate within 24 hr; room temp storage
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| Ova and parasite (O&P) | OP&P transport device (with formalin and PVA) | Patient should have not used laxatives, antacids, or stool softeners | Wait 7-10 days if pt has been taking Pepto-Bismol, Kaopectate, or MOM | Store at room temp and deliver to lab within 24 hr
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| Urine | Sterile, screw-cap container | Instruct patient in clean catch midstream collection | Hold at 4"C and deliver to lab within 24 hr | Hold at 4*C (39.2*F) and plate within 24 hr
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| Superficial wound | Aerobic transport swab | Wipe area with sterile saline or alcohol prep pad before collection | Rotate swab while gently swiping wound | Transport and plate within 24 Hr; room temperature storage
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| Deep wound or abscess | Anaerobic transport device | Wipe area with sterile saline or alcohol prep pad before collection | Aspirate material, excise tissue, or insert swab deep into wound | Store at room temperature; transport to labe within 4 hr
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| Blood | Blood culture media set or Vacutainer brand blood culture tube with SPS | Disinfect venipuncture site with alcohol swab and Betadine | Draw blood during febrile episodes (usually drawn- two sets from opposite arms) | Deliver to lab within 2 hrs; incubate at 37*C (98.6*F)
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Created by:
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