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Microbiology

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Question
Answer
Sterile refers to the complete absence of what?   all life  
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Name the most common gaseous sterilizing agent   ethylene oxide  
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How do dry heat and moist heat differ in their ability to kill microbes?   dry heat less efficient than moist heat--dry heat must reach higher temps to sterilize objects  
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Name a machine in the hospital and one in many home that produces enough steam pressure to sterilize items in less than an hour   hospitals--autoclave--home--pressure cooker  
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what are the standard pressur, temp, and time normally used to achieve sterilization with steam?   121 degrees C at fifteen pound of pressure for 15 minutes  
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What are the advantages of sterilization by gamma radiation?   does not invole heat--good for heat-sensitive materials (plastic)--gamma rays have great penetration--good for sterilizing items in sealed packages  
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What are the advantages of sterilization by ultrafiltration?   no heat/chemical reaction--good for soluble materials (enzymes/antibiotics)--good way to sterilize gases  
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What are the basic usages of disinfectants and antiseptics?   disinfectant refers to chemicals used to kill bacteria on inanimate objects--antiseptics--milder versions of disinfectants--used on human flesh  
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Differentiate among bactericide, fungicide, virucide, sporicide, and biocide   bactericides--kill bacteria--fungicides--kill fungi--virucides--kill viruses--sporicides--kill spores--biocides--nonspecific agents  
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What different effects do bacteriostatic and bactericidal agents have on bacteria?   bacteriostatic agents--do not kill bacteria--do inhibit growth--bactericidal agents--kill bacteria  
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What is the phenol coefficient?   killing capacity of any diinfectant compared to the killing capacity of phenol  
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Why have phenolics generally replaced phenol in disinfection?   phenolics--milder forms of phenols--effectively remove transient organisms from the skin without patient harm  
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How does iodine kill microbes?   iodine oxidizes proteins/enzymes--microbes become unable to synthesize macreomolecules  
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How does radiation kill microbes?   radiation ionizes and destroys DNA  
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Is 100% alcohol used as a disinfectant? What is better?   100% alcohol not used--70% solution (with water)--more effective--better at dissolving microbial membranes  
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What is pasteurization?   process of heating milk (or other drinks) high enough temp and long enough time to kill pathogens  
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Why do we use disinfectants so frequently in the clinical setting?   disinfectants uses so frequently in clinical setting to prevent nosocomial infections--since nosocomially acquired microbes often antibiotic resistant  
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How do detergents work?   remove transient microbes from surfaces--do not remove resident organisms--cationic detergents dissolve bacterial membranes  
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what kills most microbes in the stomach?   acid  
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