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Nuclear_Cytoplasmic
Nuclear & Cytoplasmic Staining
| Question | Answer |
|---|---|
| What are the two types of nucleic acids? | DNA and RNA |
| This type of nucleic acid found in the nucleus has a 5 carbon sugar called deoxyribose and its major constituent is nuclear chromatin? | DNA |
| This type of nucleic acid found in the nucleus and in the ribosomes contains a 5 carbon sugar called ribose? | RNA |
| The sugars in DNA and RNA differ by a single or a double hydroxl group? | Single |
| The stain demonstates the presence of DNA? | Feulgen Reaction |
| This stain differentiates between RNA and DNA and is also used as a connective tissue stain to identify plasma cells and immunoblasts? | Methyl Green Pyronin Y |
| This stain differentiates cells present in hematopoietic tissue? | Giemsa |
| This stain is considered the most specific to demonstate amyloid? | Alkaline Congo Red |
| This stain is a rapid screening method to demonstate amyloid? | Crystal Violet |
| This stain is a fluorescent stain to demonstrate amyloid? | Thioflavin T Fluorescent Method |
| For the fixation of this stain you can use ANYTHING except BOUIN'S? | Feulgen Method |
| This substance comprises 1-2% carbohydtrate (chrondriotin, heparin and dermatan) and acid mucopolysaccharides. | Amyloid |
| A disease characterized by an amorphorous, eosinophilic, extracelular deposits? | Amyloidosis |
| This disease gradually replaces cellular elements of vital organs and causes progressive loss of function and eventual cell death? | Amyloidosis |
| This type of amyloidosis occurs spontaneously in the absence of any predisposing disease? | Primary Amyloidosis |
| This type of amyloidosis is assocoated with predisposing diseases such as chronic inflammatory diseases? | Secondary Amyloidosis |
| Chronic inflammatory disease such as rheumatoid arthritis and ankylosing spondylitis causing infections such as tuberculosis and osteomyelitis are associated with which type of amyloidosis? | Secondary Amyloidosis |
| This type of amyloidosis is assocoated with disease of the immunological system? | Myeloma-Associated Amyloid |
| This type of amyloidosis is assocoated with many tumors, especially APUD (amine precursor uptake and decarboxylation) | Tumor Associated Amyoid |
| The organs most effected with this type of amyloidosis are muscle, heart, skin and tongue? | Primary Amyloidosis |
| The organs most effected with this type of amyloidosis are kidneys, liver, spleen and adrenal gland? | Secondary Amyloidosis |
| What color does DNA stain in the Feulgen stain for DNA? | DNA stains magenta/reddish purple |
| In the Methyl Green Pyronin Y stain, what color do DNA and RNA stain? | DNA green or blue/green. RNA red. |
| What color do nuclei and bacteria stain in the giemsa polychromatic stain? | Blue |
| For Congo Red, what color does Amyloid stain? | Amyloid stains deep pink to red with a light micropsope and bright green apple with a polarizing microscope. |
| What color does amyloid stain in the Crystal Violet stain? | Purplish/violet |
| What color does amyloid stain in the Thioflavin T Fluorescent stain? | Fluorescent yellow to yellow/green. |
| The principle of this stain is that it is a combination of acid hydrolysis and aldehyde staining? | Feulgen Reaction for DNA |
| A principle of the stain is differential staining is due to the differing degrees of polymerization between DNA and RNA? | Methyl Green Pyronin Y |
| What is the T control for Methyl Green Pyronin Y? | Section containing many plasma cells |
| What is a control for amyloid stains? | Any tissue containing amyloid |
| What are three stains that stain for amyloid? | Congo Red. Crystal Violet and Thioflavin T |
| What is the contriol for Giemsa? | Spleen |
| The principle for this stain is a dye attaches to amyloid and requires no differentiation? | Thioflavin T |
| What do the three amyloid stains cut at? | Congo Red 8-10. Cryastal Violet 10-12. Thioflavin T 6-10 |
| What fixatives are used for the three amyloid stains? | Alcohol or Carnoy is preferred for Congo Red and 10% NBF for Crystal Violet and Thioflavin T. |
| Pretreatment with Alkali aids in the relase of native internal hydrogen bonds between adjacent protein chains creating more sites for the dye to bind is a principle for what stain? | Congo Red |
| A principle of this stain is the addition of HCl in the stain prevents the cytoplasmic component from overstaining? | Crystal Violet |
| The principle of this stain is that a basophilic dye methylene blue is combined with acidic dyes eosin, azure A and azure B to create a neutral dye that demonstrates a wide variety of colors when used to stain hematopoietic cell nuclei or platelets? | Giemas for hematopoietic tissue |
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Pixel