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Microorganism Stains
| Question | Answer |
|---|---|
| Microorganism Stain that detects the presence of acid fast mycobacteria? | Kinyoun Acid-Fast Stain |
| Microorganism Stain that detects the presence of Mycobacterium Leprae? | Fite Acid Fast Stain for Leprosy Organisms. |
| Microorganism Stain that detects the presence of Mycobacterium Tuberculosis or other acid fast organisms? | Auramine-Rhodamine Fluorescence Microwave Technique |
| Microorganism Stain that detects the presence of Helicobacter Pylori? | Modified Diff-Quik Giemsa Stain for Helicobacter Pylori |
| Microorganism Stain that detects the presence of Fungi? | Gridley Fungus Stain |
| Microorganism Stain that detects the presence of fungal organisms? | Grocott Methenamine-Silver Nitrate Fungus Stain |
| Microorganism Stain that detects the presence of spirochetes? | Warthin-Starry Technique for Spirochetes |
| Microorganism Stain that detects the presence of spirochetes, helicobacter pylori, or causation agent of legionellosis? | Steiner and Steiner Microwave Procedure. |
| All of the 8 Microorganism stains are cut at what micron? | 4 to 5 microns |
| 10% NBF is preferred for all the microorganism stains and others can be used except Carnoy's on which two? | Kinyoun Acid-Fast and Fite Acid-Fast |
| Tissue containg acid-fast organisms must be used as a control for which two microorganism stains? | Kinyoun Acid-Fast and Fite Acid-Fast |
| Tissue containing AFB Mycobacterium is used as a control for which microorganism stain? | Auramine-Rhodamine Fluorescence Microwave Technique |
| Tissue containing H. Pylori is used as a control for which microorganism stain? | Modified Diff-Quik Giemsa Stain for Helicobacter Pylori |
| Tissue containing fungus is used as a control for which two microorganism stain? | Grocott Methenamine-Silver Nitrate Fungus Stain and Gridley Fungus Stain |
| Tissue containing spirochetes is used as a control for which microorganism stain? | Warthin-Starry Technique for Spirochetes |
| Tissue containing spirochetes, helicobacter pylori, or causation agent of legionellosis is used as a control for which microorganism stain? | Steiner and Steiner Microwave Procedure. |
| What color are the AFB and the background in the Kinyoun stain? | AFB is bright red and the background is light blue. |
| What color are mycobacterium leprae and AFB aas well as the background in the Fite stain? | Mycobacterium leprae and AFB are bright red and background is light blue. |
| What color are the AFB and the background in the Auramine-Rhodamine Fluorescence stain? | AFB are reddish-yellow fluorescence and background is black. |
| What color is the H pylori, bacteria and nuclei in the Diff-Qiuk Giemsa stain? What color is the cytoplasm? | Blue. Cytoplasm is pink |
| What colors are the fungus, Mucin and background in Grocott Methenamine Silver Stain? | Fungus is black, Mucin is taupe to dark gray and background is green. |
| What colors are the Mycelia, conidia, mucina nd elastic fibers in the Gridley Stain? What color is the background? | Deep-purple/rose and yellow background |
| What colors are the spirochetes and other bacteria in the Warthin-Starry Stain? What color is the background? | Black. Background is pale yelow to light brown. |
| What colors are spirochetes, helicobacter pylori, legionella pneumophilia,other baceria in Steiner and Steiner Stain? What color is the background? | Dark Brown/black and light yellow background. |
| What microrganism stain must you avoid mercurial and chromium fixatives? | Steiner and Steiner |
| Two Fluorescent basic dyes used in combination are the principle of what microorganism stain? | Auramine-Rhodamine Fluorescence |
| This stain is a Romanowsky stain which is a neutral dye due to the combination of basic methylene blue and acid eosin. | Modified Diff-Quik Giemsa |
| This microorganism stain stains for Pneumocystis as well as fungal organisms and a section containing Pneumocystis must be used as a control? | Grocott Methenamine silver Nitrate |
| This method is NOT satisfactory for the detection of Mycobacterium Leprae | Kinyoun |
| The lipoid capsule of the acid fast organism takes up the carbol fuchsin and resists decolization with dilute mineral acid in this stain? | Kinyoun |
| Use only Millipore filtered water in floatation bath and staining steps before carbol-fuchsin because tap water has been reported to have AFB+ microorgansims is a special consideration when performing which two microrganism stains? | Kinyoun and Fite |
| What three microorganism stains use a negative control cut from the same day's workload and using the same floatation bath to eliminate solution cotamination? | Kinyoun, Fite and Auramine-Rhodamine. |
| Use only Millipore filtered water in floatation bath and staining steps before auramine-Rhodamine because tap water has been reported to have AFB+ microorgansims is a special consideration when performing which microrganism stain? | Auramine-Rhodamine |
| This is a highly sensitive stain that can give false posiitives? | Auramine-Rhodamine |
| Do not prolong in the last water or in the dehydrating alcohols because excessive decolorization may occur using this stain? | Modified Diff-Quik Geimsa |
| Polysaccarides in the cell wall are oxidized to aldehydes by chromic acid. Only substances with large quantities of polysaccharides will remain active with the methamine silver reducing it to visible metallic silver? | Grocott Methenamine-Silver Nitrate |
| The principle of this stain is that Chromic acid oxidizes adjacent glycol groups to aldehydes. The aldehydes then react with the Schiff Reagents? | Gridley |
| Overheating the silver solution for this stain causes it to break down and staining may be nonspecific? | Grocott Methenamine Silver Nitrate |
| Slides using this stain can be restained with carbol-fuchsin to confirm positivity? | Auramine-Rhodamine |
| Failure to remove alcohol during hydration using this stain reduces the chromic acid changing the solution from orange to brown? | Grocott Methenamine Silver Nitrate |
| Very old, nonviable fungi are not well stained as withnthe Grocott Methamine Silver method using this stain? | Gridley |
| A less intense reaction is obtained than with the PAS technique and background staining is also decreased using this stain? | Gridley |
| These 2 stains use an argrophilic method; spirochetes have the ability to bind silver ions from a solution, but not to reduce the silver to visible metallic form. A chemical reducer, HYDROQUINONE, is used for this purpose. What | Warthin-Starry and Steiner and Steiner |
| All bacteria are nonselectively blackened by silver impregnation using this stain? | Warthin-Starry |
| This stain provides for greater sensitivity when screening for small numbers of gram+ or gram- bacteria? | Warthin-Starry |
| Removal of calcium and formalin pigments is essential when only a few organisms are present when using this stain? | Steiner and Steiner |
| Over developed sections can be docolorized with iodine and sodium thiosulfate and restained using this stain? | Warthin-Starry |
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