Question | Answer |
Bacterial proteins that cleave DNA at specific sequences | Restriction enzymes |
A palindrome and a restriction site | GAATTC |
The human ... gene is removed from human DNA and subcloned into a .... The bacteria then produce ... which can be used to treat type I and II diabetes | Insulin, Bacterial plasmid, Human insulin |
DNA is negatively charged | True |
DNA is separated on gel electrophoreis based on | Molecular weight |
Technique used to identify DNA | Southern Blot |
Technique used to identify RNA | Northern Blot |
Technique used to identify Protein | Western Blot |
A single-stranded piece of DNA or RNA that is used to visualize complementary sequences within DNA | Probe |
Utilizes dideoxy nucleotides to sequence segments of DNA | DNA sequencing |
A technique used to amplify DNA | Polymerase Chain REaction (PCR) |
Derived from teh bacteria Thermus Aquaticus commony found in hot springs. Use in PCR | Taq polymerase |
A technique used by researchers to knockout a particular gene of interest | Gene Knockout |
A technique used to identify DNA chromosomal abnormalities. For example, trisomies, monosomies, and various other abnormalities that can be detected by karyotypes. | Karyotype |
A technique used to create an exact copy of oneself. | Cloning |
Inhibitor RNAs | RNAi |
Used to inhibit protein expression by inducing the degradation of mRNA | RNAi |
The enzyme responsible for cleavage or RNAi into siRNA | Dicer |
The enzyme responsible for cleavning the endogenous mRNA | RNA Induced Silencing Complex (RISC) |
Macular Degeneration, Hypercholesterolemia, RSV, Hepatitis C | Disease currently being investigated for RNAi treatment |