micro test 3
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on it to display the answer.
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Non fermenters | Reqiure oxygen,cannot ferment glucose and form weak acids
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Cannot use carbohydrates in the absence of oxygen | Nonsaccharolytic
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Use compounds other than carbohydrates for energy | Nonoxidizers
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Nonsaccharolytic | Nonreactive on TSI slants
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detects small amount of acid,1% carbohydrate,0.2%peptones | OF medium
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Suspect of a Nonfermentor | Growth better on blood plate than MAC,oxidase pos,TSI K/K
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Detemines wheather an organism uses carbohydrate to produce acid product | OF test
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Infections occur where moisture accumulates,eyes,ears catheters | P.aeruginosa
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P.Aeroginosa | Chronic lung infection, CF,
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UTI's, pulmonary disease, endocarditis and septicemia | P.aeruginosa
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P.aeruginosa | grape like odor
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P. Aeruginosa | Large colonies,grape like odor,oxidase positive,pyocyanin
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Pseudomnas means what in greek | False unit
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Produces pyoverdin, does not grow at 42C, | P.Fluorescens and P.Putida
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P.Stutzeri | Soil denitrifier,cause of otitis media and septic arthritis
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How many species are in Burkholderia | 50 species
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B.Cepacia is found where | IV fluids,detergents and disinfectants
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Cause of onion bulb rot | B.cepacia
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Opportunistic and nosocmiaol pathogen and isolates itself from alcohol/iodine | B.CEPACIA
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Growth on Blood, chocolate and MAC | B.cepacia
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Yellowish pigment and dirt like odor | B.cepacia
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Primary in horses,goats,sheep and donkeys | B.Mallei
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Only non-motile organism in Burkholderia and Pseudomonas species | B.Mallei
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B.Psuedomallei | Aggressive granulomatous pulminary disease
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3rd most commonly isolated non-fermenter infections | Stenotrophomonas Maltophilia
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Aerobic,motile by polar flagella,resistance to antimicrobial agents | S. Maltophilia
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S.Maltophilia | Strongly oxidizes maltose, amonia odor
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Aerobic nonfermentative gram neg.bacilli,grows on MAC,oxidase neg. nonmotile | Acineobacter
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Acineobacter | Produces purple pigment on MAC
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Non-pathogenic to healthy people,most infections in immunocompromised | Acineobacter
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A.baumannii | Saccharolytic
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A.Iwoffii | Nonsaccharolytic
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Pathogen,Isolation indicates contamination/colonization rather infection | A.Iwoffii
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Found in plankton,brackish, and salt water water, borne illness | Vibrio Cholerae
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Acute diarrheal disease that is spread mainly through contaminated water | Cholera
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Attached to intestinal villi by pilli | Cholera
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Cholera Toxin or Choleragen | Massive outpouring of Rice water stools
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Vibro sp. | Natural inhabitants of seawater,Most require increased sodium chloride for growth
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Oxidase Positive, Facultative Anaerobes, Non-spore forming Gram Negative Bacilli | Vibro sp.
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Vibrio cholerae Epidemiology | Water borne illness Found in plankton, fresh, brackish,and salt water
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is an acute diarrheal disease that is spread mainly through contaminated water | Cholera
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Colonizes the gastrointestinal tract | Cholera
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Vibrio cholera 01-Two Biotypes | 1.Classic--Nonhemolytic, VP Negative2.El Tor---Beta hemolytic, VP Positive
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NLF on MAC,Yellow Colonies on TCBS Vibrio cholera | Vibrio cholera
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Vibrio parahaemolyticus | Gastroenteritis from seafood (Shell Fish),Green on TCBS, Non sucrose fermenter
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Require 1-2% NaCl for growth,Cause gastroenteritis, wound infections,septicemia | Halophilic Vibrios
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Vibrio vulnificus | Septicemia from raw shellfish,Lactose fermenter
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Aeromonas sp. | Oxidase positive, glucose fermenting, gram negative rods
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Cause diseases in warm bloodedand cold blooded animals | Aeromonas sp.
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Straight, not curved rods,single polar,Grow in range of temp from 0-42C | Aeromonas sp.
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Aeromonas hydrophilia | Water loving,Grows on MAC, Indole Positive, Waterborne Illness
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Produces aerolysin,Cytotoxic enterotoxin,Causes tissue damage in fish | Aeromonas hydrophilia
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MAC and SBA,Usually beta-hemolytic,Positive indole and oxidase | Aeromonas hydrophilia
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Aeromonas hydrophilia | resistant to Penicillin,ampicillin, carbenicillin,cephalothin
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Plesiomonas sp. | Oxidase positive, glucose fermenting, facultatively anaerobic gram negative rods
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Potential cause of enteric disease in humans, carried on cold blooded animals | Plesiomonas sp
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Causes gastritis | Plesiomonas shigelloides
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not recovered on routine media Both are small, curved, motile,gram negative bacilli | Campylobacter and Helicobacter
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S Shaped,Microaerophilic (Reduced Oxygen)Oxidase Positive and Nonfermentative | Campylobacter spp.
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are pathogenic and are associated with a wide variety of human diseases | Campylobacter spp
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Campylobacter spp | cause abortion in domestic animals such as cattle, sheep, swine
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One of the most common causes of bacterial gastroenteritis in numerous parts of US | C. jejuni
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Bloody stool with high number of WBC’s,Lasts for 7-10 days,Can be fatal | C. jejuni
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Ingestion of contaminated food or water | C. jejuni
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Special atmospheric and temperature needs:42C,5% O2, 10% CO2, 85% N2 | C. jejuni
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C. jejuni | Curved gram negative bacilli Sea gull shape
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Helicobacter spp | Curved, microaerophilic gram negative rods,Most species- strong urease
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Natural habitat is human stomach | H. pylori
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Coverts urea to ammonia,bicarbonate,Elicits a powerful immuneresponse- Ulcer | H. pylori
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Positive- Oxidase, Catalase,Urease | H. pylori
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Alcaligenes | Tiny motile gram negative rods Oxidase positive and grows on MAC
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Reduces nitrates to nitrites Found in water and soil | Alcaligenes
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Most commonly isolated species | Alcaligenes faecalis
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Alcaligenes faecalis | Opportunistic infections of blood, CSF,UTI, and wound infection
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irregular edges (feather edge) on BAP “Fruity” plate odor,Grows in 6.5% NaCl | Alcaligenes faecalis
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Small Coccobacilli,Fastidous, Obligate aerobes | Bordetella species
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B. pertussis and B. parapertussis are | non motile,
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Worldwide 60 million cases with,500,000 deaths,US occurs August to November | B. pertussis
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Exotoxin enters targetcells and activates cAMP | Pertussis toxin-
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causes ciliatedepithelial cells destruction | Tracheal cytotoxin
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a cell surface,protein binds to host cell surface | Hemoaggutination
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Whooping Cough | B. pertussis
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Potato infusion agarand sheep blood with methicillin | Bordet-Genou
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Charcoal agar with 10%Horse Blood and caphalexin | Regan-Lowe
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B. parapertussis | Causes pertussis with milder symptoms– Nontoxigenic strain
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Kennel cough in dogs, human infectionsresults from transmission from animal | B. bronchisepica
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