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micro test 3

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Question
Answer
Non fermenters   Reqiure oxygen,cannot ferment glucose and form weak acids  
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Cannot use carbohydrates in the absence of oxygen   Nonsaccharolytic  
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Use compounds other than carbohydrates for energy   Nonoxidizers  
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Nonsaccharolytic   Nonreactive on TSI slants  
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detects small amount of acid,1% carbohydrate,0.2%peptones   OF medium  
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Suspect of a Nonfermentor   Growth better on blood plate than MAC,oxidase pos,TSI K/K  
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Detemines wheather an organism uses carbohydrate to produce acid product   OF test  
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Infections occur where moisture accumulates,eyes,ears catheters   P.aeruginosa  
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P.Aeroginosa   Chronic lung infection, CF,  
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UTI's, pulmonary disease, endocarditis and septicemia   P.aeruginosa  
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P.aeruginosa   grape like odor  
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P. Aeruginosa   Large colonies,grape like odor,oxidase positive,pyocyanin  
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Pseudomnas means what in greek   False unit  
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Produces pyoverdin, does not grow at 42C,   P.Fluorescens and P.Putida  
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P.Stutzeri   Soil denitrifier,cause of otitis media and septic arthritis  
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How many species are in Burkholderia   50 species  
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B.Cepacia is found where   IV fluids,detergents and disinfectants  
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Cause of onion bulb rot   B.cepacia  
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Opportunistic and nosocmiaol pathogen and isolates itself from alcohol/iodine   B.CEPACIA  
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Growth on Blood, chocolate and MAC   B.cepacia  
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Yellowish pigment and dirt like odor   B.cepacia  
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Primary in horses,goats,sheep and donkeys   B.Mallei  
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Only non-motile organism in Burkholderia and Pseudomonas species   B.Mallei  
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B.Psuedomallei   Aggressive granulomatous pulminary disease  
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3rd most commonly isolated non-fermenter infections   Stenotrophomonas Maltophilia  
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Aerobic,motile by polar flagella,resistance to antimicrobial agents   S. Maltophilia  
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S.Maltophilia   Strongly oxidizes maltose, amonia odor  
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Aerobic nonfermentative gram neg.bacilli,grows on MAC,oxidase neg. nonmotile   Acineobacter  
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Acineobacter   Produces purple pigment on MAC  
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Non-pathogenic to healthy people,most infections in immunocompromised   Acineobacter  
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A.baumannii   Saccharolytic  
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A.Iwoffii   Nonsaccharolytic  
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Pathogen,Isolation indicates contamination/colonization rather infection   A.Iwoffii  
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Found in plankton,brackish, and salt water water, borne illness   Vibrio Cholerae  
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Acute diarrheal disease that is spread mainly through contaminated water   Cholera  
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Attached to intestinal villi by pilli   Cholera  
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Cholera Toxin or Choleragen   Massive outpouring of Rice water stools  
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Vibro sp.   Natural inhabitants of seawater,Most require increased sodium chloride for growth  
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Oxidase Positive, Facultative Anaerobes, Non-spore forming Gram Negative Bacilli   Vibro sp.  
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Vibrio cholerae Epidemiology   Water borne illness Found in plankton, fresh, brackish,and salt water  
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is an acute diarrheal disease that is spread mainly through contaminated water   Cholera  
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Colonizes the gastrointestinal tract   Cholera  
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Vibrio cholera 01-Two Biotypes   1.Classic--Nonhemolytic, VP Negative2.El Tor---Beta hemolytic, VP Positive  
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NLF on MAC,Yellow Colonies on TCBS Vibrio cholera   Vibrio cholera  
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Vibrio parahaemolyticus   Gastroenteritis from seafood (Shell Fish),Green on TCBS, Non sucrose fermenter  
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Require 1-2% NaCl for growth,Cause gastroenteritis, wound infections,septicemia   Halophilic Vibrios  
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Vibrio vulnificus   Septicemia from raw shellfish,Lactose fermenter  
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Aeromonas sp.   Oxidase positive, glucose fermenting, gram negative rods  
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Cause diseases in warm bloodedand cold blooded animals   Aeromonas sp.  
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Straight, not curved rods,single polar,Grow in range of temp from 0-42C   Aeromonas sp.  
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Aeromonas hydrophilia   Water loving,Grows on MAC, Indole Positive, Waterborne Illness  
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Produces aerolysin,Cytotoxic enterotoxin,Causes tissue damage in fish   Aeromonas hydrophilia  
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MAC and SBA,Usually beta-hemolytic,Positive indole and oxidase   Aeromonas hydrophilia  
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Aeromonas hydrophilia   resistant to Penicillin,ampicillin, carbenicillin,cephalothin  
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Plesiomonas sp.   Oxidase positive, glucose fermenting, facultatively anaerobic gram negative rods  
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Potential cause of enteric disease in humans, carried on cold blooded animals   Plesiomonas sp  
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Causes gastritis   Plesiomonas shigelloides  
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not recovered on routine media Both are small, curved, motile,gram negative bacilli   Campylobacter and Helicobacter  
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S Shaped,Microaerophilic (Reduced Oxygen)Oxidase Positive and Nonfermentative   Campylobacter spp.  
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are pathogenic and are associated with a wide variety of human diseases   Campylobacter spp  
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Campylobacter spp   cause abortion in domestic animals such as cattle, sheep, swine  
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One of the most common causes of bacterial gastroenteritis in numerous parts of US   C. jejuni  
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Bloody stool with high number of WBC’s,Lasts for 7-10 days,Can be fatal   C. jejuni  
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Ingestion of contaminated food or water   C. jejuni  
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Special atmospheric and temperature needs:42C,5% O2, 10% CO2, 85% N2   C. jejuni  
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C. jejuni   Curved gram negative bacilli Sea gull shape  
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Helicobacter spp   Curved, microaerophilic gram negative rods,Most species- strong urease  
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Natural habitat is human stomach   H. pylori  
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Coverts urea to ammonia,bicarbonate,Elicits a powerful immuneresponse- Ulcer   H. pylori  
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Positive- Oxidase, Catalase,Urease   H. pylori  
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Alcaligenes   Tiny motile gram negative rods Oxidase positive and grows on MAC  
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Reduces nitrates to nitrites Found in water and soil   Alcaligenes  
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Most commonly isolated species   Alcaligenes faecalis  
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Alcaligenes faecalis   Opportunistic infections of blood, CSF,UTI, and wound infection  
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irregular edges (feather edge) on BAP “Fruity” plate odor,Grows in 6.5% NaCl   Alcaligenes faecalis  
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Small Coccobacilli,Fastidous, Obligate aerobes   Bordetella species  
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B. pertussis and B. parapertussis are   non motile,  
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Worldwide 60 million cases with,500,000 deaths,US occurs August to November   B. pertussis  
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Exotoxin enters targetcells and activates cAMP   Pertussis toxin-  
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causes ciliatedepithelial cells destruction   Tracheal cytotoxin  
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a cell surface,protein binds to host cell surface   Hemoaggutination  
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Whooping Cough   B. pertussis  
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Potato infusion agarand sheep blood with methicillin   Bordet-Genou  
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Charcoal agar with 10%Horse Blood and caphalexin   Regan-Lowe  
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B. parapertussis   Causes pertussis with milder symptoms– Nontoxigenic strain  
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Kennel cough in dogs, human infectionsresults from transmission from animal   B. bronchisepica  
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