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AGT Lab Manual Information

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Where to find spontaneously dividing cells   bone marrow, lymph node, testis, chorionic villi of placenta, some leukemic blood, solid tumor, some pleural or ascetic fluid, fetal ascites or cystic hygroma, and often fetal or newborn blood; plus blood lymphocytes, amniotic fluid, skin and fibroblasts  
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Mitotic arresting agents   Colcemid, colchicine, or Velban  
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Who coined the words mitosis, chromatin, prophase, metaphase, anaphase, and telophase   Walther Flemming, 1879-1889  
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Who coined the word chromosome in 1888   W. Waldeyer  
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Q banding method discoverer   Caspersson in 1968: quinacrine mustard stain; allowed identification of each pair as well as small parts of each chromosome  
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Tissue mosaic syndromes   Pallister-Killian syndrome and trisomy 20 mosaicism  
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Suspension Culture tissue types   bone marrow, blood, some neuroblastomas, and most lymphomas, suspended in growth medium and do not attach to the culture vessel  
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Attached monolayer culture tissue types   amniocytes, skin fibroblasts, which require adherence to the culture vessel to grow  
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Fanconi's anemia open system gas exchange   no oxygen and may be gassed only with nitrogen (sensitive to the presence of oxygen)  
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Media with buffering systems   CO2-bicarbonate equilibrium and HEPES buffer  
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RPMI 1640 type of culture   Blood, bone marrow, solid tumor  
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RPMI 1603 type of culture   Blood, bone marrow  
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Media for all types of culture   MEM or Eagle's MEM (minimal essential medium); MEM Alpha; DME (Dulbecco's modified Eagle's); Ham's F10; Ham's F12; McCoy's 5A  
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Fragile X media   Medium 199 or TC 199 (low folic acid media)  
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Media for Amnio and CVS   Chang; Amniomax; Amniogrow (all for neoplastic cells)  
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Relative centrifugal force calculation   RCF = 0.00001118 x r(radius in cm) x Nsquared (rotating speed, RPM)  
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Hypotonic affects:   spreading, chromatid width and separation, and in bloods and bone marrows, the elimination of red blood cells  
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Colcemid concentration affects:   chromosome spreading and morphology  
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Increased dosage of Colcemid:   increases MI, straightens chromosomes, crisps chromatid edges, and increases chromosome spreading as it releases them from the mitotic apparatus  
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colchicine, a derivative of   autumn crocus  
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Velban   Vinblastine sulfate, mitotic arrestant  
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