Questions about "Smear Preparation and Simple Staining"
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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Bacterial cells are difficult to see because their index of refraction is very similar to ________. | water or air
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A good spear should appear only slightly ________. | turbid
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A slant culture is generally considered a better source of organisms for staining than is a ________ culture. | broth
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An important consideration for the slide used for staining is that it be ________. | clean
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True or false: Tap water can be used to make smears because there are too few bacteria (about 10,000 per ml) to see under oil immersion. | True.
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A smear is best made if during the heating stage (mild/high) heat is used. | mild
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True or false: Virtually everyone has bacteria in their saliva. | True.
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The number of cells cited in the introduction (500,000 per ml) will result in about 1 cell per oil immersion field when observed under the microscope. If you had ten cells per field, you would have about ________ bacteria per ml of your saliva. | 5,000,000
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The large nucleated cells observed in saliva are most likely ________. | squamous
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If cells stained with methylene blue are washed too long, the dye will be ________. | removed or washed away
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