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Questions about "Smear Preparation and Simple Staining"

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Question
Answer
Bacterial cells are difficult to see because their index of refraction is very similar to ________.   water or air  
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A good spear should appear only slightly ________.   turbid  
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A slant culture is generally considered a better source of organisms for staining than is a ________ culture.   broth  
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An important consideration for the slide used for staining is that it be ________.   clean  
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True or false: Tap water can be used to make smears because there are too few bacteria (about 10,000 per ml) to see under oil immersion.   True.  
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A smear is best made if during the heating stage (mild/high) heat is used.   mild  
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True or false: Virtually everyone has bacteria in their saliva.   True.  
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The number of cells cited in the introduction (500,000 per ml) will result in about 1 cell per oil immersion field when observed under the microscope. If you had ten cells per field, you would have about ________ bacteria per ml of your saliva.   5,000,000  
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The large nucleated cells observed in saliva are most likely ________.   squamous  
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If cells stained with methylene blue are washed too long, the dye will be ________.   removed or washed away  
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