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Review for ASCP-SM

QuestionAnswer
Children with beta hemolytic streptococci infections can develop: Acute Glomerulonephritis
Haemophilus influenzae becomes resistant to ampicillin when the organism produces a(n) beta-lactamase
A liquid fecal specimen from a 3 month old infant is submitted for microbiologic examination. In addition to culture on routine media for Salmonella and Shigella, this specimen routinely should be: examined for the presence of Campylobacter sp.
Gram positive coccus with Optochin Neg, Bacitracin Neg, Bile Esculine Neg, Hippurate hydrolysis Pos, and Catalase Neg: Group B strep (S.agalactiae)
GNB that is ox+, glucose OF:fermentative, cat+, mot-, & NG on MAC (from a cat bite) Pasteurella multocida
Bacterial infection with symptoms resembling appendicitis. Yersinia enterocolitica
Most useful morphological feature in mycelial phase of Histoplasma capsulatum tuberculate macroconidia, 8-14 micrometers
Middlebrook 7H10 & 7H11 media must be refrigerated in teh dark and incubated in the dark as well. If these conditions are not met, the media may prove toxic for mycobacteria because: formaldehyde may be produced
Ureaplasma urelyticum are difficult to grow in the laboratory on routine media because of their requirement for: ferric pyrophosphate
The cyst stage may be recovered from formed fecal specimens submitted for parasitic examination if the specimen is stored at refrigerator temperature.
stock cultures for QC of deoxyribonuclease (DNAse) production are Serratia marcescens/Escherichia coli
stock cultures for QC of deamination activity are Proteus mirabilis/Escherichia coli
stock cultures for QC of oxidase production are: E.coli/P.aeruginosa
stock cultures for QC of motility are: E.coli/K.pneumoniae
Antibiotic that inhibits cell wall synthesis is Penicillin
N.gonorrhoeae ox +, glu +, maltose -
Organism mistaken for N.gonorrhoeae in gram stain of uterine cervix exudates: Moraxella osloensis
Sodium bicarbonate and sodium citrate are components of: JEMBEC system
Advantage of antimicrobial dilution test it gives varying degrees of organism sensitivity and resistance
Clinical resistance to penicillin dosages appears to correlate with beta-lactamase production in: Neisseria gonorrhoeae
What organism is considered universally susceptible to penicillin Streptococcus pyogenes
First generation cephalosporins can be adequately represented by: cephalothin
which of teh following media is routinely used to culture Campylobacter jejuni Skirrow's medium
Which one of the following results is typical of Campylobacter jejuni optimal growth at 42 degrees celcius
Which of the following organisms must be incubated in a microaerophlic environment for optimal recovery of the organism: Campylobacter fetus
The recovery of some Cryptococcus species may be compromised if the isolation media contains cycloheximide
The one characteristic by which an unknown Cryptococcus species can be identified as Cryptococcus neoformans is: positive Niger seed agar test
A cell culture line commonly used for the recovery of Chlamydia trachomatis from clinic specimens is McCoy's
lactose neg; oxidase neg; TSI:a/a; Indole neg; urease +; Ornithine decarboxylase + ; Sucrose +; H2S neg; motility at 22 deg C positive; Yersinia enterocolitica
Autopsy on 8yr child revealed Waterhouse-Friderichsen syndrome. Blooc & throat cultures taken just prior to death were positive for what organism? Neisseria meningitidis
An anaer gnb isolated from BC following bowel surgery grew wh smooth nonhem cols. GS showed pale, bipolar-staining rod w/rounded ends. Bile stimulated growth of org and catalase was produced. Isolate resistant to Colistin, kanamycin & vanc. Indole neg: B.fragilis
Relasping fever in humans can be caused by Borrelia recurrentis
A suspension sof orgs suspected to be Shigella based on biochemical rxns didn't agglutinate with Shigella antisera subgroups A, B, C & D. The tech would conclude that: this particular Shigella possesses a capsular antigen that blocks agglutination in O antiserum and must first be destroyed by heating.
Which test is used to monitor bactericidal activity druing antimicrobic therapy in cases of endocarditis Schlichter
In a disk diffusion susceptibility test, which of the following can result if teh disks are placed on the inoculated media and left at room temperature for an hour before incubation? Zones of larger diameter would result
Which one of the following combinations of organisms would be appropriate as controls to test the indole? E.coli & P.mirabilis
Which organism could be used as teh positive quality control test for lecithinase on egg yolk agar? Clostridium perfringens
The porphyrin test was devised to detect strains of Haemophilus capable of ; synthesis of porphobilinogen
Failure to obtain anaerobiosis in anaerobe jars is most often due to the inactivation of the palladium-coated alumina catalyst pellets.
AN gncb from lung abscess-1-2mm translucent nonpig col; fluoresced brick red under ultraviolet light. Growth in bile-inhibited; Vanc Resistant, Catalase neg, Esculin hydrolysis neg, Indole neg, Nitrate Neg; glu, lac & suc-acid produced Prevotella melaniogenica
A thin anaer, gnb w/tapered ends isolated from empyema is indole pos, lipase neg, inhibited by 20% bile. Colonies were speckled or resembling ground glass & fluoresced weekly when exposed to UV light. Fusobacterium nucleatum
Which anaerobe is inhibited by sodium polyanethol sulfonate (SPS)? Peptostreptococcus anaerobius
Which is the most reliable test to differentiate Neisseria lactamica from Neisseria meningitidis? Lactose degradation
Which is the best test to differentiate Salmonella and Citrobacter species KCN, malonate, beta-galactosidase, lysine decarboxylase
Which characteristic best differentiates Acinetobacter species from Moraxella species? production of indophenol oxidase
Recent evidence has shown that specimens to be inoculated for the recovery of acid fact bacilli should be centrifuged at approximately: 3500g
The most important constituent of teh tubercle bacillus for the activity of teh various tuberculins is teh: protein
An antibiotic used to suppress or kill contaminating fungi in media is cycloheximide
What are the positive and negative controls respectively for teh germ tube test? Candida albicans and Candida tropicalis
The term "internal autoinfection" is generally used in referring to infections with Strongyloides stercoralis
When stool examination is negative, the preferred specimen to the diagnosis of paragonimiasis is sputum
Protozoan cysts are without peripheral chromatin on the nuclear membrane. Each cyst has four nuclei and each nucleus has a large karyosome that appears as a refractive dot. These oval shaped cysts are most likely: Endolimax nana
Created by: nemery12