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Review for ASCP-SM
| Question | Answer |
|---|---|
| Children with beta hemolytic streptococci infections can develop: | Acute Glomerulonephritis |
| Haemophilus influenzae becomes resistant to ampicillin when the organism produces a(n) | beta-lactamase |
| A liquid fecal specimen from a 3 month old infant is submitted for microbiologic examination. In addition to culture on routine media for Salmonella and Shigella, this specimen routinely should be: | examined for the presence of Campylobacter sp. |
| Gram positive coccus with Optochin Neg, Bacitracin Neg, Bile Esculine Neg, Hippurate hydrolysis Pos, and Catalase Neg: | Group B strep (S.agalactiae) |
| GNB that is ox+, glucose OF:fermentative, cat+, mot-, & NG on MAC (from a cat bite) | Pasteurella multocida |
| Bacterial infection with symptoms resembling appendicitis. | Yersinia enterocolitica |
| Most useful morphological feature in mycelial phase of Histoplasma capsulatum | tuberculate macroconidia, 8-14 micrometers |
| Middlebrook 7H10 & 7H11 media must be refrigerated in teh dark and incubated in the dark as well. If these conditions are not met, the media may prove toxic for mycobacteria because: | formaldehyde may be produced |
| Ureaplasma urelyticum are difficult to grow in the laboratory on routine media because of their requirement for: | ferric pyrophosphate |
| The cyst stage may be recovered from formed fecal specimens submitted for parasitic examination if the specimen | is stored at refrigerator temperature. |
| stock cultures for QC of deoxyribonuclease (DNAse) production are | Serratia marcescens/Escherichia coli |
| stock cultures for QC of deamination activity are | Proteus mirabilis/Escherichia coli |
| stock cultures for QC of oxidase production are: | E.coli/P.aeruginosa |
| stock cultures for QC of motility are: | E.coli/K.pneumoniae |
| Antibiotic that inhibits cell wall synthesis is | Penicillin |
| N.gonorrhoeae | ox +, glu +, maltose - |
| Organism mistaken for N.gonorrhoeae in gram stain of uterine cervix exudates: | Moraxella osloensis |
| Sodium bicarbonate and sodium citrate are components of: | JEMBEC system |
| Advantage of antimicrobial dilution test | it gives varying degrees of organism sensitivity and resistance |
| Clinical resistance to penicillin dosages appears to correlate with beta-lactamase production in: | Neisseria gonorrhoeae |
| What organism is considered universally susceptible to penicillin | Streptococcus pyogenes |
| First generation cephalosporins can be adequately represented by: | cephalothin |
| which of teh following media is routinely used to culture Campylobacter jejuni | Skirrow's medium |
| Which one of the following results is typical of Campylobacter jejuni | optimal growth at 42 degrees celcius |
| Which of the following organisms must be incubated in a microaerophlic environment for optimal recovery of the organism: | Campylobacter fetus |
| The recovery of some Cryptococcus species may be compromised if the isolation media contains | cycloheximide |
| The one characteristic by which an unknown Cryptococcus species can be identified as Cryptococcus neoformans is: | positive Niger seed agar test |
| A cell culture line commonly used for the recovery of Chlamydia trachomatis from clinic specimens is | McCoy's |
| lactose neg; oxidase neg; TSI:a/a; Indole neg; urease +; Ornithine decarboxylase + ; Sucrose +; H2S neg; motility at 22 deg C positive; | Yersinia enterocolitica |
| Autopsy on 8yr child revealed Waterhouse-Friderichsen syndrome. Blooc & throat cultures taken just prior to death were positive for what organism? | Neisseria meningitidis |
| An anaer gnb isolated from BC following bowel surgery grew wh smooth nonhem cols. GS showed pale, bipolar-staining rod w/rounded ends. Bile stimulated growth of org and catalase was produced. Isolate resistant to Colistin, kanamycin & vanc. Indole neg: | B.fragilis |
| Relasping fever in humans can be caused by | Borrelia recurrentis |
| A suspension sof orgs suspected to be Shigella based on biochemical rxns didn't agglutinate with Shigella antisera subgroups A, B, C & D. The tech would conclude that: | this particular Shigella possesses a capsular antigen that blocks agglutination in O antiserum and must first be destroyed by heating. |
| Which test is used to monitor bactericidal activity druing antimicrobic therapy in cases of endocarditis | Schlichter |
| In a disk diffusion susceptibility test, which of the following can result if teh disks are placed on the inoculated media and left at room temperature for an hour before incubation? | Zones of larger diameter would result |
| Which one of the following combinations of organisms would be appropriate as controls to test the indole? | E.coli & P.mirabilis |
| Which organism could be used as teh positive quality control test for lecithinase on egg yolk agar? | Clostridium perfringens |
| The porphyrin test was devised to detect strains of Haemophilus capable of ; | synthesis of porphobilinogen |
| Failure to obtain anaerobiosis in anaerobe jars is most often due to the | inactivation of the palladium-coated alumina catalyst pellets. |
| AN gncb from lung abscess-1-2mm translucent nonpig col; fluoresced brick red under ultraviolet light. Growth in bile-inhibited; Vanc Resistant, Catalase neg, Esculin hydrolysis neg, Indole neg, Nitrate Neg; glu, lac & suc-acid produced | Prevotella melaniogenica |
| A thin anaer, gnb w/tapered ends isolated from empyema is indole pos, lipase neg, inhibited by 20% bile. Colonies were speckled or resembling ground glass & fluoresced weekly when exposed to UV light. | Fusobacterium nucleatum |
| Which anaerobe is inhibited by sodium polyanethol sulfonate (SPS)? | Peptostreptococcus anaerobius |
| Which is the most reliable test to differentiate Neisseria lactamica from Neisseria meningitidis? | Lactose degradation |
| Which is the best test to differentiate Salmonella and Citrobacter species | KCN, malonate, beta-galactosidase, lysine decarboxylase |
| Which characteristic best differentiates Acinetobacter species from Moraxella species? | production of indophenol oxidase |
| Recent evidence has shown that specimens to be inoculated for the recovery of acid fact bacilli should be centrifuged at approximately: | 3500g |
| The most important constituent of teh tubercle bacillus for the activity of teh various tuberculins is teh: | protein |
| An antibiotic used to suppress or kill contaminating fungi in media is | cycloheximide |
| What are the positive and negative controls respectively for teh germ tube test? | Candida albicans and Candida tropicalis |
| The term "internal autoinfection" is generally used in referring to infections with | Strongyloides stercoralis |
| When stool examination is negative, the preferred specimen to the diagnosis of paragonimiasis is | sputum |
| Protozoan cysts are without peripheral chromatin on the nuclear membrane. Each cyst has four nuclei and each nucleus has a large karyosome that appears as a refractive dot. These oval shaped cysts are most likely: | Endolimax nana |
Created by:
nemery12
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