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MUA M2B2 IDR Assays

Bioassay methods for estimation of potency of substances by observing their effects and comparing the effect of unknowns to the effect of a standard. is the only assay to be done if an active principle of drug is unknown and if it is hard to isolate
Cell culture In vitro. The cell based in vitro assays generally use immortalized cancer cells
Organ culture in vitro
Whole animal assay in vivo. Can be used for comparison of different products intended for the same clinical indication
Direct Assay Doses of the standard and test are sufficient to produce a DIRECTLY MEASURED standard response.
Indirect assay based on quantitative response 1.relationship between the dose and response of each preparation is first ascertained. 2.Then the dose corresponding to a given response is obtained from the relation for each preparation separately
Indirect Assay based on quantal response no response or maximum response (0 or 1 – presence or absence) response such as death. These can be biossayed by end point method. Predetermined response is measured which is produced by threshold effect.
Matching method the responses obtained are matched by a trial and error process until they produce equal effects. Lacks percision and matched only at one dose. But doesn’t depend on assumed dose response relationship.
Bracketing Method selecting two standard doses, which will give a close bracket on either side of the response produced by the unknown.
Interpolation Method simplest assay graded response assay. sensitivity of the tissue is first determined by prior plotting. concentration of unknown is then read from the standard graph. Accurate
Multiple point assays Responses are repeated several times and the mean of each is taken
Antimicrobial Assay method that uses microorganisms to determine the antimicrobial potency of the antibiotics
Antifungal Assay Cylinder Plate Method, Paper-disc Method
Agar plug diffusion method is often used to highlight the antagonism between microorganisms
Minimum inhibitory concentration lowest concentration that inhibits growth
Minimal Bacteriostatic Concentration no colonies. can’t be determined until the cultures are plated in fresh media WITHOUT any antibiotics. Not routinely determined
Dose Response curve A curve obtained upon plotting the relationship between the dose of a drug administered and its pharmacological effect.
LD50 Lethal Dose 50 is the concentration of a substance which causes the death of 50% (one half) of a group of test organisms. one way to measure the short-term poisoning potential (acute toxicity) of a material
Antimitotic Assay any applied stimulus which produces a consistent change
LD01 Lethal dose for 1% of the animal test population
LD100 Lethal dose for 100% of the animal test population
LDLO The lowest dose causing lethality
TDLO The lowest dose causing a toxic effect
Methods only used on Bacteria Agar Dilution, Time- Kill Test
Adherent Culture Appropriate for most cell types, cells dissociated enigmatically, growth limited by surface area, used for cytology
Suspension Culture Appropriate for nonadhesive cells, daily cell counts required, dilution can stimulate growth, doesn't require dissociation, growth limited by cell number, used or bulk protein and batch harvesting
Created by: splashgreen