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Histology Sesh IB

Part B, Session I, Exam I, Slides 30-

Give examples of cells that secrete primarily via the regulated pathway 1. neurons 2. endocrine cells 3. most exocrine gland cells
Give examples of cells that secrete via constitutive pathway 1. fibroblasts 2. plasma cells 3. osteoblasts
List the 2 pathways for exocytosis Constitutive & Regulated
Lysosomes 1. Collections of acid hydrolases (pH 4.5) 2. Has enzymes to degrade DNA, RNA, prots, carbs, lipids 3. Detected via stains for acid phosphatase 4. Monomers released by hydrolysis released into cytosol for use by cell
Explain difference between SEM & FF-TEM 1. SEM coats entire specimen w/ metal then scans surface 2. FF-TEM freezes w/ liquid N2, cover with metal, no scanning
Hematoxylin is considered what kind of dye? What does it dye? 1. Basophilic 2. nucleus, nucleolus, RER, free polysomes
Eosin is considered what kind of dye? What does it dye? Why? 1. Acidic 2. collagen, mito, lysosomes 3. bc of its high protein content
When the sublinguinal gland is stained with H&E one specific area tends to be more pink than the rest, what is it? striated duct
When looking at the salivary gland under the microscope how do you tell the difference between the 2 diff types of secretory cells 1. Mucous cells paler 2. Serous cells darker bc the material is less soluble
What dye is good to use in order to differentiate connective tissues from other tissue? (Masson) Trichrome
Parfocal once one objective is in focus each of the others will be in focus too with little need for adjustments
How do you clean microscope lens lens tissue only
An obviously eosinophilic cytoplasm is assoc with 1. High #s mito or 2. accumumlations of acidophilic secretory vesicles
An obviously basophilic cytoplasm is assoc with Many ribos (free polysomes or accumul of RER, or mix of both
What does a smooth cytoplasm via LM possibly indicate 1. few ultra-structural features to the cytoplasm
What does a granular cytoplasm possibly indicate Clumps of secretory vesicles present or of RER & ribosomes
What does clear areas in the cytoplasm possibly indicate lipids and glyocgen have been washed away during fixation
What does a foamy cytoplasm possibly indicate presence of small lipid droplets
Micrometer = 10^-3
Millimeter = 10^-6
Meter = 1 um
Length in diameter for RBC (erythrocyte) 7-8 um
Trichrome stains are not designed to give good _____ detail nuclear
Nucleated RBCs are normal in: 1. adult birds 2. adult reptiles 3. early embryonic mammals
When looking at a giant multipolar neuron smear, what do the blue clumps in the cytoplasm represent? Accumulations of rER
Metachromasia 1. happens with dyes such as meth blue or toluidine blue 2. shifts color from itself to a red or purple
In stratified epithelia, it is the shape of what that determines the type of epithelium seen surface cells
Name 2 organs that are lined with moise (non-keratinized) stratified squamous epithelium Vagina & Esophagus
Surface cells retain their nuclei in a ______ epithelium nonkeratinized stratified squamous
3 examples of Simple Columnar Epithelium Jejunum, Colon, Fallopian tube ampulla
Ciliated epithelium can (possibly) be seen in which organ Fallopian tube
The spleen is an intraperitoneal organ and this is surfaced with ______ mesothelium
Give examples of simple squamous epithelium 1. artery, vein, nerve 2. spleen
Give examples of simple cuboidal epithelium Thyroid, Kidney
Give examples of stratified columnar epithelium 1. Penis 2. Salivary gland
Give examples of stratified cuboidal epithelium Skin (thick or thin)
Give examples of stratified columnar epithelium 1. Trachea 2. Esophagus 3. Hyaline cartilage
Give an example of Transitional epithelium Urinary bladder
What is the difference between endocrine and exocrine glands 1. Endocrine glands: ductless, secrete their hormones into blood stream via CT around the gland 2. Exocrine: secrete their product onto a surface, uni or multicellular
Give an example of a unicellular exocrine gland goblet cell (seen in resp epith, intestine, conjunctive of eye)
In multicellular glands, if end is shaped like tube it is called ______, flask it is called _______, sac-like dilataion it is called ______ tubular; acinar/alveolar, tubuloalveolar
Mucous gland vs Serous gland 1. Mucous gland: makes carb-rich material, nucleus pushed far 2. Serous gland: makes watery, prot-rich material, nucleus not pushed far
Intraepithelial glands 1. may be unicellular (like goblet cell) or multicell sheets (mucous cells lining stomach)
Intramural glands 1. glands within wall of an organ but deep to the epithelium
Extramural glands 1. located beyond visceral wall 2. such as liver, pancreas
I am lo­cated near the cell apex and seal off the intercellular space, allowing the epithelium to isolate certain body compartments. Who am I? Zonula occludens
The deeper layer of the dermis is the reticular layer
How can you remember that the reticular layer is deeper in the dermis than the papillary layer? Ret sounds like rats which can be found deep underground like where the penguin lives
Papillary layer is ______ CT and Reticular layer is ______ CT loose irregular; dense irregular
Created by: NinaP183