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Review of Amino Acid Metabolism

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Question
Answer
3 sources of amino acids are:   1. Body Protein 2. Dietary Protein 3. Synthesis of non-essential amino acids  
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When does our body use protein as a source of energy?   When we have excess protein or a lack of Carbs and Lipids.  
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List the essential amino acids:   Phe, Val, Thr, Ile, Met, His, Arg, Lys, Leu  
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What amino acids are formed by transamination reactions?   Alanine, aspartate, and glutamate  
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Alanine is formed from ______ using the enzyme ______.   Pyruvate; ALT (with glutamate as a cofactor)  
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Aspartate is formed from ______ using the enzyme ________.   Oxaloacetate; AST  
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Asparagine is formed from _______.   Aspartate  
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Glutamine is formed from ________.   Glutamate  
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In the formation of asparagine, _________ is the amide donor.   Glutamine  
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Proline is formed from _______.   Glutamate  
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Serine is formed from ________.   3-phosphoglycerate (intermediate of glycolysis)or glycine  
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Glycine is formed from _________.   Serine  
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Cysteine is formed from _______.   Serine and Methionine  
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Tyrosine is formed from _________.   Phenylalanine  
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PKU is a disease that leads to CNS problems and pigmentation deficiencies and is caused by_____.   The absense of phenylalanine hydroxylase; the enzyme that catalyzes the conversion of phenylalanine to tyrosine.  
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Homocystinuria is a disease that causes premature vascular disease and mental defects and is caused by ________.   An absence of the enzyme cystathionine synthase which catalyzes the creation of Cystine.  
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3 sources of amino acids are:   1. Body Protein 2. Dietary Protein 3. Synthesis of non-essential amino acids  
🗑
When does our body use protein as a source of energy?   When we have excess protein or a lack of Carbs and Lipids.  
🗑
List the essential amino acids:   Phe, Val, Thr, Ile, Met, His, Arg, Lys, Leu  
🗑
What amino acids are formed by transamination reactions?   Alanine, aspartate, and glutamate  
🗑
Alanine is formed from ______ using the enzyme ______.   Pyruvate; ALT (with glutamate as a cofactor)  
🗑
Aspartate is formed from ______ using the enzyme ________.   Oxaloacetate; AST  
🗑
Asparagine is formed from _______.   Aspartate  
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Glutamine is formed from ________.   Glutamate  
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In the formation of asparagine, _________ is the amide donor.   Glutamine  
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Proline is formed from _______.   Glutamate  
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Serine is formed from ________.   3-phosphoglycerate (intermediate of glycolysis)or glycine  
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Glycine is formed from _________.   Serine  
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Cysteine is formed from _______.   Serine and Methionine  
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Tyrosine is formed from _________.   Phenylalanine  
🗑
PKU is a disease that leads to CNS problems and pigmentation deficiencies and is caused by_____.   The absense of phenylalanine hydroxylase; the enzyme that catalyzes the conversion of phenylalanine to tyrosine.  
🗑
Homocystinuria is a disease that causes premature vascular disease and mental defects and is caused by ________.   An absence of the enzyme cystathionine synthase which catalyzes the creation of Cystine.  
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Ketogenic amino acids yield ______.   acetyl CoA or acetoacetate  
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Glucogenic amino acids yield ______.   Pyruvate or Kreb cycle intermediates  
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Aminotransferases (transaminases) depend on   Vitamin b6  
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The 2 most important aminotransferases are:   ALT and AST  
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Alanine amino transferase (ALT) changes alanine and a-ketoglutarate into _________   pyruvate and glutamate  
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Aspartate amino transferase (AST) changes glutamate and a-ketoglutarate into ________   Aspartate and oxaloacetate  
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_________ are the sources of urea nitrogen.   Aspartate and ammonia  
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________ releases free ammonia for urea synthesis.   Oxidative deamination reaction of glutmatate.  
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The oxidative deamination reaction of glutamate releases what products?   a-ketoglutarate + NH3 (ammonia)  
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What enzyme is involved in the oxidative deamination of glutamate?   glutamate dehydrogenase  
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Where is arginase found and what does it do?   Arginase is only found in the liver; It converts arginine to urea in the Urea cycle.  
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What is the overall reaction of the Urea Cycle?   Aspartate + NH3 + CO2 + 3 ATP ----> Urea + Fumurate + 2 ADP + AMP + 2 Pi + PPi  
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The first two reactions of the Urea cycle occur in the ________, and the remaining three take place in the __________.   Mitochondrial Matrix; Cytosol  
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