Immuno Lec 6
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IgG | Highest concentration in serum.
In tissue and blood
longest half life.
Important for passive immunization.
Crosses placenta
Activates Complement
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IgM | In Blood.
monomer in B cell, pentamer in serum.
Most important compliment activator.
Blood borne infections.
half life - 5 days.
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IgA | monomer in serum
dimer in secretions, mucosal surfaces.
J chain is in dimeric, secreted by B cell
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Transcytosis of IgA across epithelium | IgA binds poly Ig receptor, endocytosis of both, goes to lumen, now IgA and poly Ig receptor are cleaved enzymatically.
Secretory IgA has part of the poly Ig receptor on it.
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IgD | Marker for mature B cell
very low conc in serum
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IgE | lowest serum conc, shortest half life.
bind to mast cells via its FC region located below epithelial surfaces, respi tract, and GI
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Effector Functions of B cells | carried out as a consequence of Ab/Ag binding. most mediated through Fc region.
Neturalization, opsonization, ADCC, mast cell degranulation, and complement.
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Neutralization | IgG and IgA.
Ab binds Ag, neutralizes function. Ag can no longer bind receptor. prevents toxin action, virus entry, and bacterial adhesion.
Basis of Passive immunization
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Active Immunization | induction of an immune response by injection of an Ag.
requires several weeks to months. long lasting immunity.
have lag phase. activate specific T and B cells and memory.
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Passive Immunization | Transfer of immunity by injection of Ab.
Neutralization.
give when immediate risk of exposure.
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Opsonization | Ab mediated Phagocytosis.
Carried out by macrophages and neutrophils.
Major mechanism for destruction of encapsulated bacteria.
Main isotype is IgG.
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Mechanism of Opsonization | Abs bind to target pathogen, once they bind, conformational change occurs in Fc region. Now recognizable by Mac and neutrophils.
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ADCC | Antigen Dependent Cell Mediated Cytotoxicity. By eosinophils and NK cells. Destruction of antibody coated cells.
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NK cells- ADCC | Self cell that is infected, may be cancerous. MHC1 on surface. Ab recognizes MHC1. NK binds FCy receptor, releases toxic granules.
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Eosinophil - ADCC | Eosinophil - receptor for IgE on surface. IgE - parasitic infections. Too big for macs/B cells. IgE binds parasite, change FC region, eosinophils bind to FC receptor now. release toxic mediates, induce inflammatory response.
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Mast cell degranulation | Mast Cells, coated with IgE by FC region. Many. It is preloaded, waiting. If binds parasitic infection, crosslinking occurs and degranulation occurs of histamine and serotonin. Increase Vasc Permeability, blood flow.
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Complement System | Three pathways. classical, alternate, lectin. consequences are membrane damage and lysis of pathogen or release of mediators for opsonization and inflammation.
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Classical Complement Pathway | Ag/Ab binds to bacteria. C1q needs to bind to two FC regions of IgM or IgG. q->r->s, C1s cleaves C4 and C2. a= active, b = binding.
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Classical Complement Cascade | Ag+Ab+C1s = AgAbC1s complex.
C1s cleaves C4 and C2. C4b+C2b = C3 convertase. Cleaves C3, C3b+c4b+c2b = C4b2b3b, C5 convertase. cleaves C5, C5b= initates membrane attack complex.
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What initiates membrane attack complex? | C5b
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What do all the a sections do? | recruit inflammatory cells
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Role of C3b | Can activate opsonization or can join to become c5 convertase.
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Formation of Membrane Attack Complex | C5 into lipid bilayer, t hen c6, c7, c8, multiple copies of C9 make up the pore, then influx of fluids and molecules.
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Alternate Pathway | Innate. Ab surface is enough.
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Alternate Pathway Activation + Cascade | C3(labile). Small amts cleaved. C3b in serum.c3b+microbe = C3b complex. C3b complexes with factor B, factor D cuts B. now C3bBb which is c3 convertase. Stabilized by properitin. now c3bBbP3b , C5 convertase.
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Lectin Pathway | Innate. acts as opsonin. acute phase proteins produced in response to IL-6.
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Lectin Pathway Activation. | MBL(mannin binding leptin)=acute phase protein.
MBL + MASP + CHO complex. Cleaves C4 and C2. Now you have C4b2b(C3 convertase). Add C3b, and you have c5 convertase. then follows classical pathway.
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Cell surface proteins that regulate complement activity | Bind c3b or Bind C8.
c8 = mac inhibitory factor.
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Serum proteins that regulate complement activity | c1 inhibitor(prevent spontaneous activation)
Factor H and I - bind c3b(enzymatic degradation)
Properdin - required for alternate pathway, stabilizes C3bBb.
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Complement Proteins Also opsonins | C3b and C4b
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Complement Proteins also anaphylatoxins | c3a, c4a, c5a
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Complement Proteins also chemotaxins | c3a, c5a
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Inherited immunodeficiency | specific genetic defect
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acquired immunodeficiency | secondary to an underlying disordder. malnutrition, aging, viral infection. etc.
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X-linked agammaglobulinemia - Bruton's | no circulating B cells.
BTK defect , B cells halted at Pre-B cell. increase susceptibility to extracellular encapsulated organisms.
replacement therapy of gamma globulin.
passively immunize them.
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Antibody levels in infants | at 6 months, you have low IgG because maternal transferred IgG goes away. normal IgM and IgA. more pronounced in prematures. respiratory tract infections.
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Selective IgA deficiency | Low IgA. other classes normal/elevated.
increased allergies, autoimmunity
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X-linked hyper-IgM | elevated IgM. only trace levels of the rest.
Tcell defect, no CD40L.
no class switching.
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Created by:
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