Stack #56157
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| Haemophilus influenzae | small coco bacilli satelite growth around other colonies on BAP respiratory droplets spread infection
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| H. influenzae | encapsulated- life threatening meningitis, b serotype non-encapsulated- locaolized infections
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| Haemophilus ducrei | not normal flora STD- chanhroid ulcer, tropics look like school of fish on slide
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| Haemophilus aegyptius | eye infection long rods
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| Haemophilus | gram neg rods, pale pink, capnophiles(CO2) normal flora urinary tract
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| Special growth requirements | Hemin=factor X, NAD=factor V, nicotine adenine dinucleotide, temp & moisture sensitive, plate immediately
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| Pasteurella | gram negative small bacilli rods, non-motile, glucose fermentors
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| Pasteurella tests | oxidase, indole, urease positive
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| Pasteurella multocida | not normal flora, may be normal flora of animal handlers, infection from dog scratch/bite, treat with PCN
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| pasteurella tests | urease negative, grows well on BAP & Choc agar
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| Mannheimia haemolytica | grows well on BAP & Choc agar, indole & urease negative
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| Suttonella indologenes | rare eye infection
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| Bisgaerds taxonella | dog bite wound
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| CBC group EF-4a | bite wounds
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| Campyl, Arco & Heliobacter | small moitle curved rods, gram stain faintly pink, majority are pathogens
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| Campyl, Arco & Heliobacter | found in GI tract & reproductive tract of animals, poultry major source, majority are pathogens
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| Syndromes of Campyl & Arcobacter | febrile systemic disease-fever, periodontal disease, GI disease- most common, arco spp, does not multipy in food but may in water
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| C. jejuni | raw milk, undercooked poultry, contaminted waste
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| jejuni | AIDS pats can cause meningitis & endocarditis, post infection complication-Guillan-Barre syndrome
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| Specimen-Campyl, Arco & Heliobacter | fecal or blood, Cary-Blair medium 4 transport
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| Plate-Campyl, Arco & Heliobacter | skirrows agar, Campy agar, columbia agar w/lysed horse serum
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| Morph-Campyl, Arco & Heliobacter | seagull wing, direct exam
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| Helicobacter pylori | human stomach, biopsy, grows slowly
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| Test-Helicobacter pylori | urease, catalase & oxidase positive
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| Helicobacter pylori cont | treat with triple drug therapy, relapses occur, serodiagnostic tests
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| Legionnairres Disease | Legionella pneumophila, Phila 1976, fatal 10-20% casea
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| Morph-Legionnairres Disease | gram negative rod, stains faintly pink/red thin, giemsa or silver stain as backup
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| Cont-Legionnairres Disease | media must contain L-cysteine & a pH 6.9, need deep sputum specimen
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| Plate-Legionnairres Disease | BYCE-buffered charcoal yeast extract, w/polymixin B, anisomycin, cetamandole to fill any fungus
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| Location-Legionnairres Disease | found in lakes, rivers, marine water, moist soils; man made- AC ducts, cooling towers, humidifiers, hot tubs
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| Trans-Legionnairres Disease | not transmitted from person-2-person, can live outside the cell & in macrophages, needs a moist environment
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| Antigen-Legionnairres Disease | respiratory secretions, DFA-direct fluorecence antibody
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| BYCE_Legionnairres Disease | gray/white to blue/green; glistening convex, circular; cut-glass apperance
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| Treat-Legionnairres Disease | resistant to PCN; erythromycin first or bactrim, rifampin, zithromax
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| Bordetella pertussis | human only host, whooping cough; paratussi less severe, bronchiseptia-normal animal flora; is an abligate aerobe
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| Test-Bordetella pertussis | gram negative, faintly staining, enhance w/safranin O counterstain; coccibacilli-single or pairs; oxidase & urease positive; grows BAP &MAC; non-motile
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| Causes-Bordetella pertussis | human urt infections; attaches to respiratory ciliated epithelial cells-adhesins; paralyze beating cilia w/tracheal toxin; Pertussis Toxin
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| Catarrhal stage | cold symptoms for weeks
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| Paroxysmal stage | violent cough for 1-4 weeks
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| Convalescent stage | symptoms slowly decrease, 6 months post infection
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| Specimen-Bordetella pertussis | nasopharyngeal swab during cough or aspirates; DFA stain w/polyclonal AB
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| Treat-Bordetella pertussis | erythromycon
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| Prevent-Bordetella pertussis | vacine, need booster shot; DPT
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