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media123

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Question
Answer
Selective for Salmonella; Differential for lactose   Brilliant Green  
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Inhibits most enteric gram negative bacteria   Brilliant Green  
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Brilliant Green Lactose Positive Color (decr. pH)   yellow/green/inhibited  
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Brilliant Green Lactose Negative Color   pink-white with red zone  
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Nonselective & differential for hemolysis   Sheep blood agar  
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Contains tryptone soybean protein & 5% sheep blood nutrient   Sheep blood agar  
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Supports all but the most fastidious organisms   Sheep blood agar  
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Enriched with hemoglobin, hemin (X factor), and coenzyme nicotine adenine dinucleotide (V factor)   Choco agar  
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Grows on blood agar and choco agar   N. gonorrhaeae  
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most gram pos & gram neg will grow   Sheep blood agar  
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most gram pos & gram neg will grow   Choco agar  
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Chocolate agar (only)   H. influenzae  
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Selective for gram positive bacteria; differential on the basis of hemolysis   CNA agar  
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Peptone nutrient, sheeps blood   CNA agar  
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Inhibit gram negative bacteria in CNA agar   Colistin & Nalidixic acid  
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Ability to grow staph & strep   CNA agar  
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demonstrates hemolysis (alpha & beta)   CNA agar  
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Inhibits bacteria besides Staph & Strep   CNA agar  
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Eosin Methylene Blue Agar   EMB  
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Selective for enteric gram negative rods; differentes lactose pos & neg   EMB  
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Dyes that inhibit gram positive bacteria on EMB   Eosin Y & methylene blue  
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EMB Lactose positive color   Dark purple center  
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Lactose positive occurs with:   Decrease pH  
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EMB Lactose negative color   No dark purple center  
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Lactose negative   Shigella  
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Lactose negative   Samonella  
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Lactose negative   Proteus  
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Stool specimen   Hektoen Enteric Agar  
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Very similar to Brilliant Green   Hektoen Enteric Agar  
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Selective for Salmonella, Differentiates Lactose positive & lactose negative; Differentiates H2S pos & neg   Hektoen Enteric Agar  
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Hektoen Enteric agar - dye that inhibits most normal enteric bacteria   Bromthymol blue & acid fuchsin (pH indicators)  
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H2S differential iron source   Ferric ammonium citrate  
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Hektoen Enteric L+   Salmon/yellow colonies  
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Hektoen Enteric L-   Green,blue  
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Hektoen Enteric H2S+   Black center in colony  
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Mac Conkey (similar)   EMB (similar)  
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Differential based on lactose; Selective for gram negative rods   Mac Conkey  
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Mac Conkey specimens   stool, sputum, urinary  
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Mac Conkey dye that inhibits gram + bacteria   crystal violet dye  
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Mac Conkey Lactose + color   Red  
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Mac Conkey Lactose - color   colorless  
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Selective for Staph; Differential due to Mannitol Salt   Mannitol Salt agar  
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Selective for Staph   Mannitol Salt agar  
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Mannitol Salt agar - what inhibits all gram - & all gram + but Staph   Na Cl  
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Differentiates wh/ Staph ferment Mannitol & wh/ don't   Mannitol  
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Ferments Mannitol color   Yellow colonies with yellow zones  
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Nonfermentor of Mannitol color   Pink  
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Phenylethyl Alcohol Agar   PEA  
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PEA (similar)   CNA (similar)  
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Selective for Staph & Strep; Differential due to hemolysis   Phenylethyl Alcohol Agar  
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Inhibits gram negative bacteria in PEA   Phenylethyl alcohol  
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Selective for Strep (Beta hemolysis)   SXT agar  
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Sheep blood agar with trimethoprim-sulfa   SXT  
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Inhibits most bacteria other than Group A & Group B strep   SXT/TMP/smx  
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Beta hemolysis   S. pyogenes & S. agalactiae  
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Stool Specimen   Selenite F Broth  
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Toxic for normal enteric flora   Sodium selenite  
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Enhances enteric pathogens - especially salmonella   Selenite broth  
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Salmonella (good growth); Shigella (partial or complete inhibition)   Selenite broth  
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Selective for N. gonorrhoeae   Thayer Martin Agar  
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Chocolate agar + antibiotics   Thayer Martin Agar  
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Thayer Martin - Inhibit other gram negative   Colistin  
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Thayer Martin - Inhibit gram positive   Vancomycin  
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Thayer Martin - Inhibit yeast   Nystatin  
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Modified Thayer Martin (MTM)   Trimethoprim - inhibits proteus  
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Normally sterile body sites   Thioglycollate broth  
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Permits growth of anaerobes   Thioglycollate broth  
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Permits growth of anaerobes   Thio, cystine (lowers oxidation)  
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Enhance the growth of pathogens that are present in small numbers   Thioglycollate broth  
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Enhance the growth of most bacteria   Beef protein, glucose  
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Xylose, Lysine - Desoxycholate Agar   XLD  
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Differential due to lactose & H2S; Selective for Salmonella & Shigella   Xylose Lysine - Desoxycholate Agar  
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XLD - Inhibits gram + bacteria & some enterics   Na desoxycholate & salts  
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XLD Lactose positive color   Yellow colonies  
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XLD Lactose negative color   Pale pink colonies  
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XLD - H2S   Ferric ammonium citrate  
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XLD - H2S +   Black center  
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XLD - Pink colonies with black center   Salmonella  
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XLD - Pink colonies with no black center   Shigella  
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Salmonella   Lactose negative  
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Salmonella   H2S positive  
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Shigella   Lactose negative  
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Shigella   H2S negative  
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E. coli   Lactose positive  
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E. coli   H2S negative  
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