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| Algorithm |
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| Bug |
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| Gram + cocci, catalase +, coagulase + |
S. aureus |
| Gram + cocci, catalase +, coagulase -, novobiocin sensitive |
S. epidermidis |
| Gram + cocci, catalase +, coagulase -, novobiocin resistance |
S. saprophyticus |
| Gram + cocci, catalase -, β hemolytic, bacitracin sensitive |
S. pyogenes |
| Gram + cocci, catalase -, β hemolytic, bacitracin resistant |
S. agalactiae |
| Gram + cocci, catalase -, α, β, or γ hemolytic, grows in 40% bile (Group D), grows in 6.5% NaCl |
E. faecalis |
| Gram + cocci, catalase -, α, β, or γ hemolytic, grows in 40% bile (Group D), susceptible to 6.5% NaCl |
S. bovis |
| Gram + cocci, catalase -, α hemolytic, bile-esculin negative, optochin susceptible |
S. pneumoniae |
| Gram + cocci, catalase -, α hemolytic, bile-esculin negative, optochin resistant |
S. mutans, S. intermedius |
| Gram + bacilli, spore-forming, aerobic, motile |
B. cereus |
| Gram + bacilli, spore-forming, aerobic, non-motile |
B. anthracis |
| Gram + bacilli, spore-forming, obligate anaerobe, motile |
C. tetani, C. botulinum, C. difficile |
| Gram + bacilli, spore-forming, obligate anaerobe, non-motile |
C. perfringens |
| Gram + bacilli, non-spore-forming, motile |
L. monocytogenes |
| Gram + bacilli, non-spore-forming, non-motile |
C. diptheriae |
| Gram + beaded filaments, not acid fast, obligate anaerobe |
A. israeli |
| Gram + beaded filaments, weakly acid fast, obligate aerobe |
N. asteroides, N. brasiliensis |
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