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Help!
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| What do you wear when working with blood, body fluids or mucous membranes? | Gloves
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| Two requirements for working in the lab. | Hair tied back and lab coat.
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| What does the biohazard symbol indicate? | Procedures marked with the biohazard icon should be performed carefully, to minimize the risk of trannsmitting the disease.
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| What is the procedure for cleaning up a spill in the lab? | Cover spilled microbial cultures with paper towels and disinfect the towels. Leave for 20 min and then clean up.
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| What disinfectant is used to clean up microbial spills? | Lysol IC
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| How does a student deal with broken glass in the lab? | Do not touch with your hands, use a broom and dustpan. Place broken glassware contaminated with microbial cultures or bodily fluids in the "to be autoclaved" container.
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| What do you always do before leaving class? | Put away equipment, lock drawer and clean desk surface.
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| What are the four objectives on the compound microscope? | 4X - 10X - 40X - 100X
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| Which is the only objective you use oil on? | 100X
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| Why do you use oil immersion? | To increase resolution.
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| How does oil on the slide help resolution? | When oil is placed between the slide and the lense, the light ray continues without refraction. Light loss is minimized. Lens is closest to the slide.
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| What are the basic shapes of bacteria? | Bacillus - rods. Coccus-circles. Spirillum-spiral.
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| What is the benefit to microscope objectives being parfocal? | When you change from one objective to the next, you only need to make small focus adjustments.
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| Who was the first individual to observe living microbes in suspension? | Antoni Van Leeuwenhoek
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| What is brightfield microscopy used for? | To observe stained and unstained organisms. Outward appearance and some internal structure.
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| True movement | independent motion for relatively long stretches.
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| False positive | Movement when there shouldn't be. (Currents)
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| False negative | Should be movement but there isn't. (Damaged flagella, bacteria dead, overcrowded)
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| What does it mean to be heterotrophic? | An organism requiring complex organic food in order to grow. (Rely on others for food)
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| Protozoa - Prokaryotic or Eucaryotic? | Eukaryotic
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| Are protozoans heterotrophic or autotrophic? | Mostly heterotrophic
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| What is the advantage of using an agar slant? | Surface area
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| What is the advantage of using a broth culture? | Bigger numbers of bacteria
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| What is the advantage of using Petri plates? | You can visualize the colonies.
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| What is the primary use of slants? | provide a solid growth surface
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| What is the primary use of broths? | Provide large number of bacteria in a small space and are easily transported.
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| What is dark field microscopy used for? | light, unstained specimens
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| What is phase-contrast microscopy used for? | live, unstained specimen for internal structure
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| What is flourescence microscopy used for? | rapid I.D. of specific organisms.
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| What is transmission electron microscopy used for? | To examine layers of specimens.
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| What is scanning electron microscopy used for? | To study surface structures of intact cells, viruses.
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Created by:
lisaanne11