| Flap 1 | Flap 2 |
| micro pages 28-32 | (blank) |
| q. What is the minimum distance that can exist between two points such that the points are observed as separate entities known as? | a. Resolving Power or resolution. |
| q. What is the maximum sesolving power of the best compound Microscope(light microscope) which is sufficient to see most bacterial structures but too low to see viruses? | a. 0.02 um |
| q. What is the maximum magnification of a compound microscope? | a. 1000X |
| q. What type of microscope is used to view living organisms and internal organs can be easily seen with? | a. Phase Contrast Microscope. |
| q.What kind of microscope appears as three diminsional images. | a. Interference microscope. |
| q. What highlights specimens against a dark background and can detect Treponema pallidum( causative agent of syphilis)? | a. Dark field microscope |
| q. What kind is used to examine materials that emit light when light of a different wavelength strikes them? | a. Flourescence Microscopy |
| q. What kind of microscope provides three dimensional pictures of thick structures such as a community of microorganisms and provides detailed sectional views of the interior of an intact cell? | a. Confocal scanning laser microscope. |
| q. What uses a beam of electrons guided by strong magnets, has resolving power of 0.3nm, generally magnifys objects 10,000x-100,000x? | a. Electron Microscope |
| q. what has the highest resolving power? | a. Transmission electron microscope.1,000,000x + |
| q.What kinds of images do Scanning electron microscopes create? | a. 3D surface structures but no internal structures. |
| q. What are the four steps to the Gram Stain? | a. 1. Primary stain 2. Mordent 3. Decolorizer 4. Counter or secondary stain. |
| q. What kind of stains are Gram stains and Acid fast stains? | a. Differential stains. |
| q. Acid fast stains are used to stain members of genus Mycobacterium due to a high lipid concentration of mycolic acid in cell wall, what are the four steps? | a. 1. steamer 2. primary dye 3. decolorizer 4. counter stain(Methylene blue). |
