| Question |
Answer |
| What is the Michaelis Menten equation? |
v=Vmax [S] / [S] + Km |
| Define Vmax. |
The rate of the reaction at saturation substrate concentrations for a given amount of enzyme. |
| The rate of the reaction at saturation substrate concentrations for a given amount of enzyme defines what concept? |
Vmax |
| Define Km. |
The substrate concentration that results in 50% Vmax. |
| The substrate concentration that results in 50% Vmax defines what? |
Km |
| An inhibitor that binds to the active site and competes with the substrate for its occupation is called a _________ inhibitor. |
Competitive inhibitor |
| An inhibitor that binds to, and takes an enzyme out of commission is called a ____________ inhibitor. |
Noncompetitive inhibitor |
| Competitive inhibitors ____________ (increase / decrease) the apparent Km. |
Increase |
| In a Lineweaver Burke plot, a competitive inhibitor will have _______________ (same / different) point on the x axis. |
Different |
| In a Lineweaver Burke plot, a noncompetitive inhibitor will have _______________ (same / different) point on the x axis. |
Same |
| In a Lineweaver Burke plot, a competitive inhibitor will have _______________ (same / different) point on the y axis. |
Same |
| In a Lineweaver Burke plot, a noncompetitive inhibitor will have _______________ (same / different) point on the y axis. |
Different |
| This type of inhibitor binds to the active site and is acted upon by the enzyme just as if it was a normal substrate. The enzyme uses its own catalytic machinery to inactivate itself. |
Suicide inhibitor |
| Fluorouracil is what type of inhibitor? |
Suicide inhibitor |
| The Y axis on the Lineweaver Burke plot indicates ___________. |
1/Vmax |
| The X axis on the Lineweaver Burke plot indicates ____________. |
-1/Km |
| The Y axis on the Eadie-Hofstee plot indicates _________. |
Vmax |
| The X axis on the Eadie-Hofstee plot indicates _________. |
Vmax/Km |
| The slope of the Eadie-Hofstee plot indicates __________. |
-Km |
| True or False: Both competitive and noncompetitive inhibition is reversible. |
True |
| True or False: Both competitive and noncompetitive inhibition is irreversible. |
False |
Biology Lab Midterm
