| Question |
Answer |
| Selective for Salmonella; Differential for lactose |
Brilliant Green |
| Inhibits most enteric gram negative bacteria |
Brilliant Green |
| Brilliant Green Lactose Positive Color (decr. pH) |
yellow/green/inhibited |
| Brilliant Green Lactose Negative Color |
pink-white with red zone |
| Nonselective & differential for hemolysis |
Sheep blood agar |
| Contains tryptone soybean protein & 5% sheep blood nutrient |
Sheep blood agar |
| Supports all but the most fastidious organisms |
Sheep blood agar |
| Enriched with hemoglobin, hemin (X factor), and coenzyme nicotine adenine dinucleotide (V factor) |
Choco agar |
| Grows on blood agar and choco agar |
N. gonorrhaeae |
| most gram pos & gram neg will grow |
Sheep blood agar |
| most gram pos & gram neg will grow |
Choco agar |
| Chocolate agar (only) |
H. influenzae |
| Selective for gram positive bacteria; differential on the basis of hemolysis |
CNA agar |
| Peptone nutrient, sheeps blood |
CNA agar |
| Inhibit gram negative bacteria in CNA agar |
Colistin & Nalidixic acid |
| Ability to grow staph & strep |
CNA agar |
| demonstrates hemolysis (alpha & beta) |
CNA agar |
| Inhibits bacteria besides Staph & Strep |
CNA agar |
| Eosin Methylene Blue Agar |
EMB |
| Selective for enteric gram negative rods; differentes lactose pos & neg |
EMB |
| Dyes that inhibit gram positive bacteria on EMB |
Eosin Y & methylene blue |
| EMB Lactose positive color |
Dark purple center |
| Lactose positive occurs with: |
Decrease pH |
| EMB Lactose negative color |
No dark purple center |
| Lactose negative |
Shigella |
| Lactose negative |
Samonella |
| Lactose negative |
Proteus |
| Stool specimen |
Hektoen Enteric Agar |
| Very similar to Brilliant Green |
Hektoen Enteric Agar |
| Selective for Salmonella, Differentiates Lactose positive & lactose negative; Differentiates H2S pos & neg |
Hektoen Enteric Agar |
| Hektoen Enteric agar - dye that inhibits most normal enteric bacteria |
Bromthymol blue & acid fuchsin (pH indicators) |
| H2S differential iron source |
Ferric ammonium citrate |
| Hektoen Enteric L+ |
Salmon/yellow colonies |
| Hektoen Enteric L- |
Green,blue |
| Hektoen Enteric H2S+ |
Black center in colony |
| Mac Conkey (similar) |
EMB (similar) |
| Differential based on lactose; Selective for gram negative rods |
Mac Conkey |
| Mac Conkey specimens |
stool, sputum, urinary |
| Mac Conkey dye that inhibits gram + bacteria |
crystal violet dye |
| Mac Conkey Lactose + color |
Red |
| Mac Conkey Lactose - color |
colorless |
| Selective for Staph; Differential due to Mannitol Salt |
Mannitol Salt agar |
| Selective for Staph |
Mannitol Salt agar |
| Mannitol Salt agar - what inhibits all gram - & all gram + but Staph |
Na Cl |
| Differentiates wh/ Staph ferment Mannitol & wh/ don't |
Mannitol |
| Ferments Mannitol color |
Yellow colonies with yellow zones |
| Nonfermentor of Mannitol color |
Pink |
| Phenylethyl Alcohol Agar |
PEA |
| PEA (similar) |
CNA (similar) |
| Selective for Staph & Strep; Differential due to hemolysis |
Phenylethyl Alcohol Agar |
| Inhibits gram negative bacteria in PEA |
Phenylethyl alcohol |
| Selective for Strep (Beta hemolysis) |
SXT agar |
| Sheep blood agar with trimethoprim-sulfa |
SXT |
| Inhibits most bacteria other than Group A & Group B strep |
SXT/TMP/smx |
| Beta hemolysis |
S. pyogenes & S. agalactiae |
| Stool Specimen |
Selenite F Broth |
| Toxic for normal enteric flora |
Sodium selenite |
| Enhances enteric pathogens - especially salmonella |
Selenite broth |
| Salmonella (good growth); Shigella (partial or complete inhibition) |
Selenite broth |
| Selective for N. gonorrhoeae |
Thayer Martin Agar |
| Chocolate agar + antibiotics |
Thayer Martin Agar |
| Thayer Martin - Inhibit other gram negative |
Colistin |
| Thayer Martin - Inhibit gram positive |
Vancomycin |
| Thayer Martin - Inhibit yeast |
Nystatin |
| Modified Thayer Martin (MTM) |
Trimethoprim - inhibits proteus |
| Normally sterile body sites |
Thioglycollate broth |
| Permits growth of anaerobes |
Thioglycollate broth |
| Permits growth of anaerobes |
Thio, cystine (lowers oxidation) |
| Enhance the growth of pathogens that are present in small numbers |
Thioglycollate broth |
| Enhance the growth of most bacteria |
Beef protein, glucose |
| Xylose, Lysine - Desoxycholate Agar |
XLD |
| Differential due to lactose & H2S; Selective for Salmonella & Shigella |
Xylose Lysine - Desoxycholate Agar |
| XLD - Inhibits gram + bacteria & some enterics |
Na desoxycholate & salts |
| XLD Lactose positive color |
Yellow colonies |
| XLD Lactose negative color |
Pale pink colonies |
| XLD - H2S |
Ferric ammonium citrate |
| XLD - H2S + |
Black center |
| XLD - Pink colonies with black center |
Salmonella |
| XLD - Pink colonies with no black center |
Shigella |
| Salmonella |
Lactose negative |
| Salmonella |
H2S positive |
| Shigella |
Lactose negative |
| Shigella |
H2S negative |
| E. coli |
Lactose positive |
| E. coli |
H2S negative |
Buen Viaje lv.2 ch 8
