| Question |
Answer |
| hematoxylin is what kind of stain? |
a dark blue or purple dye; anything that stains with hematoxylin is called basophilic |
| eosin is what kind of a stain? |
an acidic dye that stains basic components red or pink; structures that stain with eosin are called acidophilic or eosinophilic |
| why do the nuclei in cells stain blue? |
because of the heterochromatin (ionized phosphate) that resides within it |
| what color is the nucleolus? |
basophilic |
| silver stain is for what kind of tissues? |
nervous tissue, reticular fibers (type III collagen) |
| which stain stains glycogen & various carbohydrates with aldehyde? |
PAS |
| what is the goal of immunocytochemstry? |
visualize some component in a tissue section by means of an antibody |
| what structures does hematoxylin stain? |
acidic structures (DNA & RNA) |
| which H & E stain is more specific? |
hematoxylin |
| is the ECM basophilic or eosinophilic? |
eosinophilic |
| what stain is used for elastic fibers that don't stain well with H & E? |
elastic stain |
| elastic stain stains fibers what color? |
purple or black |
| which stain is used to stain collagen? |
masson's trichrome (also called connective tissue technique) |
| trichrome stain stains nuclei what color? cytoplasm? connective tissue? |
the nuclei appear blue, the cytoplasm a bright red, and the connective tissue aqua bluegreen |
| tissues or cells that stain with a silver salt are referred to as what? |
argyrophilic |
| PAS stains what color? |
dark magenta |
| why do basement membranes stain magenta with PAS? |
proteoglycans |
| PAS is commonly used to look at what structure? |
bowman's capsule |
| these stains cause tissue to take on a different color than the dye: |
metachromic stains |
| what is the most common artifact in slides? |
shrinkage |
Beef Production
