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Micro Lab Quiz #1

#1

QuestionAnswer
What do you wear when working with blood, body fluids or mucous membranes? Gloves
Two requirements for working in the lab. Hair tied back and lab coat.
What does the biohazard symbol indicate? Procedures marked with the biohazard icon should be performed carefully, to minimize the risk of trannsmitting the disease.
What is the procedure for cleaning up a spill in the lab? Cover spilled microbial cultures with paper towels and disinfect the towels. Leave for 20 min and then clean up.
What disinfectant is used to clean up microbial spills? Lysol IC
How does a student deal with broken glass in the lab? Do not touch with your hands, use a broom and dustpan. Place broken glassware contaminated with microbial cultures or bodily fluids in the "to be autoclaved" container.
What do you always do before leaving class? Put away equipment, lock drawer and clean desk surface.
What are the four objectives on the compound microscope? 4X - 10X - 40X - 100X
Which is the only objective you use oil on? 100X
Why do you use oil immersion? To increase resolution.
How does oil on the slide help resolution? When oil is placed between the slide and the lense, the light ray continues without refraction. Light loss is minimized. Lens is closest to the slide.
What are the basic shapes of bacteria? Bacillus - rods. Coccus-circles. Spirillum-spiral.
What is the benefit to microscope objectives being parfocal? When you change from one objective to the next, you only need to make small focus adjustments.
Who was the first individual to observe living microbes in suspension? Antoni Van Leeuwenhoek
What is brightfield microscopy used for? To observe stained and unstained organisms. Outward appearance and some internal structure.
True movement independent motion for relatively long stretches.
False positive Movement when there shouldn't be. (Currents)
False negative Should be movement but there isn't. (Damaged flagella, bacteria dead, overcrowded)
What does it mean to be heterotrophic? An organism requiring complex organic food in order to grow. (Rely on others for food)
Protozoa - Prokaryotic or Eucaryotic? Eukaryotic
Are protozoans heterotrophic or autotrophic? Mostly heterotrophic
What is the advantage of using an agar slant? Surface area
What is the advantage of using a broth culture? Bigger numbers of bacteria
What is the advantage of using Petri plates? You can visualize the colonies.
What is the primary use of slants? provide a solid growth surface
What is the primary use of broths? Provide large number of bacteria in a small space and are easily transported.
What is dark field microscopy used for? light, unstained specimens
What is phase-contrast microscopy used for? live, unstained specimen for internal structure
What is flourescence microscopy used for? rapid I.D. of specific organisms.
What is transmission electron microscopy used for? To examine layers of specimens.
What is scanning electron microscopy used for? To study surface structures of intact cells, viruses.
Created by: lisaanne11
 

 



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