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Micro Quiz 4/25

What is media? Substance to grow bacteria
What are four basic ingredients in media? A. Water B. Salt/ Sodium Chloride C. Peptones D. Buffers E.Solidifying agent F Indicators G Selective Agents H Enrichment
What ingredient converts liquid to a solid medium? Augar
Describe Selective media Uses dyes, antibiotics and others. Inhibts growth of certain organisms and permits growth of others. Used for fecal, urine, sputum, bacteria normally present inhibited so pathogens, if present, can be seen. EXAMPLE:strep augar
Describe differential media uses dyes, indicators and others Gives colonies of a particular organism a distinct and easily recognizable appearance EXAMPLE: E. Coli
Describe Enriched media Contains complex organic materials that certain fastidious organisms require to grow
What are the four solutions used to do a Gram stains? A. Crystal violet- primary stain B. Iodine- mordant- sticks in dye C. Acetone/alcohol- decolorizer- strips out dye D. Safranin-counter stain
What color is Gram positive? Purple
What color is Gram negative? Red
What do the first and second names of microbes denote? 1st- Genus 2nd-species
What shape are cocci? Round
What shape are bacilli? Rod Shape
What shape are spirilla? Sprial
Describe cell wall Varies in composition, accounts for different stain reactors. Rigid structure, maintains shape, some protection
Describe plasma membrane Semi permiable- regulates passage of materials in and out of cell wall
Describe nucleoid Area that stores genetic material. DNA and RNA. Not a true nucleus.
Describe a capsule A capsule is a sticky or gummy envelope secreted by some bacteria. Protects from phagocytosis associated with virulence mucoid growth on the augar
Describe flagella Aids in locomotion, propels cell
Describe spore/endospore Thick walled oval body. Resistant to adverse conditions, such as, heat, chemicals, drying, boiling for hours and freezing for 6 months
Define Aerobe Will grow in presence of free oxygen
Describe anaerobe Can not grow in presence of free oxygen. Obtains oxygen from other oxygen containing molecules
Describe facultative anaerobe Sometimes=facultative, having the ability to do a thing but not usually doing it. Basically an aerobe but can grow in the absence of free oxygen
Define obligate anaerobe Can grow only in complete absence of free oxygen
What pH is best for bacterial growth? 7- tolerates narrow range 6-8
What is the best time to use bacterial cultures for lab studies? 18-24 hrs 37 degrees Celsius
What are exotoxins? Diffuse from the bacterial cell into surrounding tissue
What is the Gram reaction of bacteria that produce exotoxins? Gram pos- Example- diphtheria, tetanus, gang green, botulism
What are endotoxins? Bound to the cell wall, released when cell distroyed
What is the Gram reaction of bacteria that produce endotoxins? Gram neg- Example- typhoid, cholera
Created by: lecopple1