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Clin Path

Neutrophils, WBC's and macrophage are a sign of ___ Inflammation
Cytology are done to differentiate ___ from ___ Inflammation from neoplasia
Histopathology: evaluates cellular architecture
Cytology: evaluates cells individually/groups
___ or ___ are used to collect swab cytology samples Sterile cotton or rayon cells
___ swabs are used for anarobic samples Rayon swabs
Vaginal swabs are taken from ____ to ____ in the vagina cranial to urethral orifice
____ and ____Cells exfoliated from the vaginal wall are passed from the _____ *neutrophils and epithelia *uterus
___ are the only cytology's that are heat fixed ear cytology
Scraping smears are useful for collecting cells from ___ but only contain ____ *firm lesions *superficial
___ shaped eosinaphils are only seen in felines rod
___ cytology's are prepared from external lesions from living animals or tissue samples Imprint
Scraping procedure: Hold scalpel blade perpendicular to sample and scrape several times, transfer to slide and spread like peanut butter
Imprint procedure: remove blood/tissue fluid by blotting, make multiple imprints on several slides
Fine needle biopsy's can be a ____ or ____ procedure Aspiration or non-aspiration
FNA use ___g needles and ___ml syringe *21-25g needle *3-20ml syringe
The softer the aspiration tissue the ___ the needle and syringe smaller
When redirecting the needle Constants ______ is necessary when using the FNA technique negative pressure
___g needle is moved through the same tract ___ times and repeated ___ times for a NON- aspirate technique. *22G needle * 5-6 times *2-3 times
No ____ is used during the Non-aspirate technique. A ___ml syringe is used *negative pressure *10 ml
____ biopsy's are not ____ before procedure and are used for cytologic /histopathologic exams. *Tisuue Biopsy's *scrubbed
Tissue samples for Histo are fixed in ___, the fixation of choice *10 % formalin
Histo sample slabs are to be no more than ___ wide and fixed in formalin at a rate of ___ times the specimen's volume *1 cm wide *1o times
Endoscopy biopsy's are flushed with ___ from tip of endoscopy sterile saline
Wedge Biopsy's are taken from a ____ zone to ___ tissue *transition *normal
Wedge biopsy's are placed on a ___ with the ___ facing up and the ___ facing down * splint *superficial tissue *deep tissue
Most common punch biopsy's are _, _, and _mm. __ is the only one that doesn't require sutures *4-6-8 *4mm
Centesis: Introduction of a needle into any body cavity/organ to remove fluid
Synovial, cerbrospinal, and adequeous fluid all require ____ for collection General anesthesia
Before collecting peritoneal and pleural fluid ____ prepare the site and equipment aseptically
__g needle and __ml syringe is the most common used needle for centesis *21g needle *60ml syringe
Needle is introduced into the ____ abdomen ___of the midline __cm caudal to umbilicus for abdominocentesis *ventral *right *1-2cm
Exudate: Thick with wbc's, rbc's, puss
Transudate: clear or staw colored, primarily protein and H20
peripheral blood contamination and recent hemorrhage result in ___ supernatant and __ sediment *clear *red
Recent hemolysis results in ___ supernatant reddish
2 types of Transtracheal/bronchial are: percutaneous or Orotracheal
insertion of needle into trachea through crycothyroid to collect fluid: Percutaneous
Placing ET tube to collect fluid via catheter: Orotracheal
Bronchoalveolar lavage is used to collect sample from the _____ lower respiratory tract
Smear prep for solid masses: Compression, combination, starfish
Fluid sample are collected into ___ tubes *EDTA tubes
___ Smears are similar to a blood smear except the slide in raised ___ through the smear and ___ smears are identical to blood smears *Line smear *3/4 through *Wedge
Preferred fixative for cytology is ______ and should remain in fixative for __min *95% methanol *2-5
Malignancy must meet ___ or more nuclear criteria 3
Primary types of tumors: Epithelial, Mesenchymal, round cell
Epithelial tumor: Aka carcinoma or adeoncarcinoma, highly cellular in clumps
Mesenchymal tumors: Aka sarcoma, less cellular/wispy spindles
Round cell tumors: Histocytoma, lymphoma, mast cell, plasma cell, and melanoma
Neoplastic cells that spread from other body tissues to the lymph nodes: Metastasis
malignant neoplasia of the lymph nodes is known as ___ lymphoma
Gram negative robs are normal flora of the ___ GI tract
The largest cells seen in a vaginal cytology are superficial cells
fully cornified cells are ____ anuclear
vaginal cytology's are a ___ assay ENDOCRINE
Cells with a polygonal shape and smal N:C ratio: Large intermediate
round/oval cell with prominent nuclei small intermediate
RBC's are seen in large numbers during ____ Proestrus
Neutrophils are in large numbers during____and absent during____ *diestrus *estrus
bacteria is seen in every stage of estrous but especially during ___ Estrus
Anestrus cells seen: Parabasal, neutrophils (NO rbc'S)
Cells seen in Proestrus: lrg int, superficail, RBC's and neutrophils
Proestrus can last __ wks 2-3
___ stage is when breeding will occur Estrus
Cells seen in Estrus: anuclear (superficial)minimal Rbc's
cells seen in Diestrus: parabasal, intermediate, abundant debris
Inflammation resulting in a pinkish-milky discharge and large numbers of neutrophils: Vaginitis/Metritis
equipmemt and supplies used for semen should be warmed to ___deg F 98.6
3 portions of ejaculate: sperm free, sperm rich and sperm poor
3 sections of a sperm: head, mid piece, tail
Created by: brit_2112