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Clinical Laboratory

Lab Questions from N.S.

What are the Standard Form (SF) series numbers laboratory request forms? The 500 series.
Where are laboratory reports of patients placed? Reports of inpatients are placed in the inpatient health record, while laboratory reports on outpatients are placed in the outpatient health record.
With what exception, SF laboratory forms are multicopied and precarbonized for convenience? The SF-545, Laboratory Report Display
Where are the carbon copies of the laboratory report forms filed? In the laboratory's master file.
What directive contains a complete listing of all standard forms (SF) and their purposes? Manual of the Medical Department, (MANMED), NAVMED P-117
What form is used to request a urinalysis test? SF-550, Urinalysis Request Form.
What part of the SF-550 Urinalysis Request Form would you enter "Clean catch midstream to R/O urinary tract infection," if applicable? In the "Remarks" block.
How should a standard form that is used to record test results, be attahed to the SF-545, Laboratory Record Display, inside the patient's health record? In chronological order.
What are two principal methods of obtaining blood samples? Finger Puncture and Venipuncture.
What part of the microscope is the the platform on which a preparation is placed for examination? The stage (of the framework).
What should NEVER be used to clean microscope lenses? Alcohol or similar solvent.
What are the 5 test involved in a complete blood count? Total RBC count; hemoglobin determination; hematocrit reading; total white blood cell (WBC) count; and the differential WBC.
Which RBCs should NOT be counted in the manual Sahli Pipette blood count method? Those touching the lines on the right and at the bottom.
What is needed to manually count red blood cells (erythrocytes) and white blood cells (leukocytes)? A microscope and an instrument called a hemacytometer.
What is the hemacytometer? A thick glass slide with three raised parallel platforms on the middle third of the device.
What is the composition of the central platform of the hemacytometer? It is subdivided by a transverse groove to form two halves, each wider than the two lateral platforms and separated from them and from each other by moats.
What hemacytometer grid is used by most laboratories for counting blood cells? The Improved Neubauer Ruling.
How are counts of RBCs and WBCs each expressed? As concentration: cells per unit volume of blood.
Why is the unit of volume for cell counts expressed as cubic millimeters? Because of the linear dimensions of the hemacytometer chamber.
What is the total red blood cell (erythrocyte) count? The number of red cells in one cubic millimeter of blood
What method is used to manually count red blood cells? The Unopette Method.
What is used in the Unopette procedure for manually counting red blood cells? A disposable diluting pipette system that provides a convenient, precise, and accurate method for obtaining a red blood cell count.
What materials are needed to perform the Unopette Method of counting red blood cells? A disposable Unopette, hemacytometer and coverglass, microscope with light source, hand-held counter, and a laboratory chit.
What is the first step in the Unopette procedure for counting red blood cells? Puncture the diaphragm in the neck of the diluent reservoir with the tip of the capillary shield on the capillary pipette.
When is filling of the Unopette pipette complete and will stop automatically? When the blood reaches the end of the capillary bore in the neck of the pipette.
In the Unopette Method for counting red blood cells, how much blood is discarded from the pipette, before loading the counting chamber of the hemacytometer? The first three drops.
What action is taken when counting cells in each field that touch the lines on the right or at the left bottom of the hemacytometer? Don't count them.
What is indicated in counting red blood cells if each field of the hemacytometer varies by more than 20? Probably indicates a poor distribution of the cells in the fluid, resulting in an inaccurate count, and the test must be repeated.
What is the formula for determining the number of RBCs per cubic millimeter of blood when using the hemacytometer? Total the number of cells counted in all five fields and multiply by 10,000.
What is hemoglobin's main function in the body? To carry oxygen from the lungs to the tissues and to assist in transporting carbon dioxide from the tissues to the lungs.
What is hemoglobinometry? The measurement of the concentration of hemoglobin in the blood.
Where does the formation of hemoglobin take place? In the developing red cells located in bone marrow.
How does dehydration affect hemoglobin? It results in above-normal hemoglobin values.
What is the most widely used automated method for testing for hemoglobin values? The cyanmethemoglobin method.
What are the manual methods for determining blood hemoglobin? The Haden-Hausse and Sahli-Hellige methods.
What determines the percentage of RBCs in whole blood? The hematocrit or packed cell volume (PCV).
What is the most accurate manual method of determining the hematocrit or packed cell volume? The microhematocrit method.
What is the first step in performing the microhematocrit method of determining packed cell volume? Fill the capillary tube two-thirds to three-quarters full with well-mixed, oxalated venous blood or fingertip blood.
Created by: AZNFlush