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Microbiology
Stack #56157
| Question | Answer |
|---|---|
| Haemophilus influenzae | small coco bacilli satelite growth around other colonies on BAP respiratory droplets spread infection |
| H. influenzae | encapsulated- life threatening meningitis, b serotype non-encapsulated- locaolized infections |
| Haemophilus ducrei | not normal flora STD- chanhroid ulcer, tropics look like school of fish on slide |
| Haemophilus aegyptius | eye infection long rods |
| Haemophilus | gram neg rods, pale pink, capnophiles(CO2) normal flora urinary tract |
| Special growth requirements | Hemin=factor X, NAD=factor V, nicotine adenine dinucleotide, temp & moisture sensitive, plate immediately |
| Pasteurella | gram negative small bacilli rods, non-motile, glucose fermentors |
| Pasteurella tests | oxidase, indole, urease positive |
| Pasteurella multocida | not normal flora, may be normal flora of animal handlers, infection from dog scratch/bite, treat with PCN |
| pasteurella tests | urease negative, grows well on BAP & Choc agar |
| Mannheimia haemolytica | grows well on BAP & Choc agar, indole & urease negative |
| Suttonella indologenes | rare eye infection |
| Bisgaerds taxonella | dog bite wound |
| CBC group EF-4a | bite wounds |
| Campyl, Arco & Heliobacter | small moitle curved rods, gram stain faintly pink, majority are pathogens |
| Campyl, Arco & Heliobacter | found in GI tract & reproductive tract of animals, poultry major source, majority are pathogens |
| Syndromes of Campyl & Arcobacter | febrile systemic disease-fever, periodontal disease, GI disease- most common, arco spp, does not multipy in food but may in water |
| C. jejuni | raw milk, undercooked poultry, contaminted waste |
| jejuni | AIDS pats can cause meningitis & endocarditis, post infection complication-Guillan-Barre syndrome |
| Specimen-Campyl, Arco & Heliobacter | fecal or blood, Cary-Blair medium 4 transport |
| Plate-Campyl, Arco & Heliobacter | skirrows agar, Campy agar, columbia agar w/lysed horse serum |
| Morph-Campyl, Arco & Heliobacter | seagull wing, direct exam |
| Helicobacter pylori | human stomach, biopsy, grows slowly |
| Test-Helicobacter pylori | urease, catalase & oxidase positive |
| Helicobacter pylori cont | treat with triple drug therapy, relapses occur, serodiagnostic tests |
| Legionnairres Disease | Legionella pneumophila, Phila 1976, fatal 10-20% casea |
| Morph-Legionnairres Disease | gram negative rod, stains faintly pink/red thin, giemsa or silver stain as backup |
| Cont-Legionnairres Disease | media must contain L-cysteine & a pH 6.9, need deep sputum specimen |
| Plate-Legionnairres Disease | BYCE-buffered charcoal yeast extract, w/polymixin B, anisomycin, cetamandole to fill any fungus |
| Location-Legionnairres Disease | found in lakes, rivers, marine water, moist soils; man made- AC ducts, cooling towers, humidifiers, hot tubs |
| Trans-Legionnairres Disease | not transmitted from person-2-person, can live outside the cell & in macrophages, needs a moist environment |
| Antigen-Legionnairres Disease | respiratory secretions, DFA-direct fluorecence antibody |
| BYCE_Legionnairres Disease | gray/white to blue/green; glistening convex, circular; cut-glass apperance |
| Treat-Legionnairres Disease | resistant to PCN; erythromycin first or bactrim, rifampin, zithromax |
| Bordetella pertussis | human only host, whooping cough; paratussi less severe, bronchiseptia-normal animal flora; is an abligate aerobe |
| Test-Bordetella pertussis | gram negative, faintly staining, enhance w/safranin O counterstain; coccibacilli-single or pairs; oxidase & urease positive; grows BAP &MAC; non-motile |
| Causes-Bordetella pertussis | human urt infections; attaches to respiratory ciliated epithelial cells-adhesins; paralyze beating cilia w/tracheal toxin; Pertussis Toxin |
| Catarrhal stage | cold symptoms for weeks |
| Paroxysmal stage | violent cough for 1-4 weeks |
| Convalescent stage | symptoms slowly decrease, 6 months post infection |
| Specimen-Bordetella pertussis | nasopharyngeal swab during cough or aspirates; DFA stain w/polyclonal AB |
| Treat-Bordetella pertussis | erythromycon |
| Prevent-Bordetella pertussis | vacine, need booster shot; DPT |
Created by:
dodge1500
on 2006-11-16