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Micro Lab Part 1

Lab 12 Physiological Characteristics of Bacteria

QuestionAnswer
What is the function of bacterial exoenzymes? Extracellular degradation of substances so that siimpler compounds can be taken into the cell as nutrient or energy resources
Differentiate between anabolism and catabolism Anabolism- energy driven synthesis of complex molecules from simpler ones. Catabolism is the energy-releasing degradatin of complex molecules into simpler ones
Differentiate between fermentation and respiration Fermentation metabolizes sugars in the absence of oxygen. Respiratin utilized oxygen.
How are acids or alkalies detected in culture mediums? By the use of pH indicators
Name two tests for reactions that produce acid end products; Sugar fermentation and fat hydrolysis
Name two tests for reactions that produce alkaline end products Urea hydrolysis, Citrate utilization, proteolysis (litmus milk & kligers iron agar)
What types of gases can be produced as a result of sugar fermentation? Carbon dioxide & hydrogen gas produced by fermentation
How are gases detected in fermentation reactions? In a durham tube as a trapped air bubble or as an air pocket in a stabbged solid medium eg Kligers Iron agar.
Name a nonfermentative test in which gas production indicates a positive test result Catalase test yields oxygen gas
The tests for the hydrolysis of starch, casein and triglycerides are similar in terms of setup. How doe the methos for the detection of hydrolysis differ between them? Casein-shows clear line of hydrolysisStarch Agar- iodine is added to form complexes and reveal clear zones of hydrolysis and Fat Hydrolysis- Spirit Blue agar contains pH indicator that reveals the release of fatty acids (by product of hydrolysis)
In addition to the casein hydrolysis test, name two other tests that demonstrate hydrolysis of proteins, or proteolytic digestion Litmus milk adn alkaline reaction in Kligers iron agar
Kligers iron agar and SIM are multiple test media what test do these media have in common? hydrogen sulfide production
What components of the Kligers and SIM media are included for this test? Cystein, ferrous salts
Both Kligers and SIM are stabbed, but for different reasons; SIM is stabbed to detect motility. Kliglers Iron Agar is stabbed to creat an aerobic environment to test for glucose.
What does Kligers Iron Agar test for? Carbohydrate fermentation and hydrogen sulfide production
What does MR-VP broth test for? 2,3 butanedoil fermentation
What does phenol red lactose test for? carbohydrate fermentation
What does SIM Medium test for? hydrogen sulfide production; tryptophan degradation and motility
What does Simmon's citrate agar test for? Citrate utilization
What does skim milk agar test for? Casein hydrolysis
What does spirit blue agar test for? triglyceride hydrolysis
What does tryptone broth test for? tryptophan degradation
Match reagent alpha naphtol w/test 2,3 butanediol fermentation
Match reagent dimethyl-alpha-naphthylamine w/test nitrate reduction
Match reagent ferric chloride w/test phenylalanine deamination
Match reagent Gram's iodine w/test starch hydrolysis
Match reagent hydrogen peroxide w/test catalase test
Match reagent Kovac's reagent w/test tryptophan degradation
Match reagent methyl red with test mixed-acid fermentation
Match reagent NNNN with test oxidase test
Match reagent potassium hydroxide with test 2,3 butanediol fermentation
Match reagent sulfanilic acid w/test nitrate reduction
Match ezyme amylase w/reaction starch hydrolysis
Match cysteine desulfurase w/reaction hydrogen sulfide production
Match lipase w/reaction triglyceride hydrolysis
Match protease w/reaction gelatin liquefaction, casein hydrolysis
Match tryptophanase w/reaction indole
Match urease w/reaction urea hydrolysis
Match product 2.3 butanediol w/ reaction Voges-Proskauer test
Match product ammonia w/reaction urea hydrolysis
Match product fatty acids w/reaction triglyceride hydrolysis
Match product indole w/reaction tryptophan degradation
Match molecular oxygen w/reaction catalase
Match phenylpyruvic acid phenylalanine deamination
Created by: Donna Pannullo Donna Pannullo on 2010-03-22



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