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Exam One

Microbiology Lecture

Bacteriology? Phycology? Mycology? Protozoology? Parasitology? Virology? Microbial Metabolism? 1. study of bacteria/Archaea. 2. study of Algae. 3. study of Fungi. 3. study of Protozoan. 4. study of parasitic protozoa and parasitic animals. 5. Biochemistry: chemical reactions in cells.
Microbial Genetics? Environmental Microbiology? Serology? Immunology? Epidemiology? 1. study of RNA/DNA. 2. study of microbes/organisms in their environment. 3. study of antibodies in serum. 4. study of the body's defense against specific diseases. 5. study of frequency, distribution and spread of diseases.
Etiology? Infection Control? Chemotherapy? Bioremediation? Public Health Remediation? 1. study of the cause of diseases. 2. promoting hygiene and controlling nosocomial infections. 3. drugs to treat an infection. 4. use of microbes to remove pollutants. 5. sewer, water purification and control of insects that spread diseases.
Agricultural Microbiology? Food and Beverage Technology? Pharmaceutical Microbiology? Recombinant DNA Technology? 1. use of microbes to control pest. 2. reduce/eliminate microbes from food/drinks. 3. create vaccines and antibiotics. 4. alternate microbial genes to synthesize useful products.
Prokaryotes? Types?(2) Eukaryotes? Types?(4) Both cells? Prokaryotes: no nucleus or organelles. Types: bacteria and archaea. Eukaryotes: have nucleus and organelles. Types: Protozoa, Arthropods, Helminths, Fungi and Algae. Both: have cell membranes cytoplasm and genetic DNA.
Acellular Infectious Agents Types?(3) Non-photosynthetic Types? Photosynthetic Types?(1) Acellular Types: Viruses, Viroid and Piron. Non-photosynthetic Type: Fungi Photosynthetic: Cyanobacteria.
Unicellular Cells? Multicellular Cells? Acellular Agents? Unicellular: Bacteria, Archaea, Algae, Fungi(yeast), Protozoa Multicellular: Algae, Fungi(mold), Helminths
Macroorganisms? Types? Microorganisms? Types? Infectious Agents Types? Macro: organisms large enough to not be seen with microscope. Types: Arthropods, Helminths Micro: organisms needed to be seen with scope. Types: Bacteria, Archaea, Fungi, Algae, and Protozoa Infectious: Virus, Pirons and Viroids
Dark Field Microscope? Bright Field Microscope? 1. see living, colorless(white), unstained organisms. dark background/bright specimen. 2. see killed, stained specimens and counting the microorganism.
Fluorescence Microscope? Confocal Microscope? 1. localize specific structures and used for diagnosis tool. dark background/bright fluorescent specimen. 2. shows details of structures with different dyes. structures are stained with specific structures.
Phase Contrast Microscope? Differential Interference Contrast Microscope? 1. observe internal structures. the specimen has light and dark areas. 2.observe internal structures. image is 3-D.
Transmission Electron Microscope? Scanning Electron Microscope? 1. detail of internal structures in bacteria and viruses. 2-D image. 2. surface detail. 3-D image.
Scanning Tunneling Probe Microscope? Atomic Force Probe Microscope? 1.observe surface of objects at atomic and molecular level. 2. observe living specimens at molecular and atomic level.
Simple Stain? single dye use, to observe the size, morphology and arrangement of the specimen. results in a purple or blue color.
Gram Stain? uses 2 dye to determining the difference between 2 large groups of microorganism. Resulting in pink for negative and purple for positive.
Acid Fast Stain? Endospores Stain? 1. highlights Mycobacteria and Nocardia. results in pink-red for acid fast or blue for non-acid fast. 2.shows endospores form the Bacillus and Clostridium Genera. results in green for endospores and pink-red for vegetation.
Negative Stain for Capsules? Flagellar Stain? 1. shows bacterial capsules. results in black background with white specimen. 2. shows the number of flagellum. results in visible flagellum.
Taxonomy? Classification? Strains? 1. identify, classify and name organisms. 2. organize large diversity of organisms. 3. parts of species.
Hierarchy of Taxa? Duhh, Keep Pot Clean Or Family Gets Sick! Domain(based on rRNA 1.eukaryote 2.bacteria 3.archaea), Kingdom, Phylum, Class, Order, Family, Genus, Species.
Scientific Names? Formula? 1.refer to the same species. 2. Take Genus (capitalized), Use Specific Epithet(lowercase) and italicize(Online Test *hint) if written underline.
Heat Fixation? Chemical Fixation? Resolution? Refraction? 1. passing a slide smear up on a Bunsen burner. 2. applying a chemical like methyl to the smear. 3. distinguishing objects that are close together. 4. bending of magnification.
Created by: aneshia