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Lab003
Chemical Methods of Control: Disinfectants and Antiseptics
| Question | Answer |
|---|---|
| After incubating the diluted chemicals and bacteria overnight, you notice there was no growth around the chemical. How can you tell if the chemical was bacteriocidal (killed organism) or bacteriostatic (simply stopped organism from growing)? | subculture organism to a sterile medium minus the chemical. If bactericidal>no growth. If bacteriostatic>growth. |
| In the use-dilution test, a chemical is evaluated by its ability to kill 10^6 to 10^8 dried Clostridium sporogenes or Bacillus subtilis endospores. Why is this considered a stringent test? | Chemicals do not readily penetrate bacterial endospores, and the number of endospores used is higher than would be expected in normal use of a disinfectant. |
| Keep in mind that you are testing the chemicals ability to fight organisms in your sink or bathtub. How accurate are your results? Why? | The results are artificial because a test tube and a sink or bathtub are not directly comparable. In a "real" situation, chemicals that alter the effect of the compound may be present. |
| What is the purpose of the use-dilution method? | to evaluate a chemical under conditions matching its application. |
| What did we use to dilute the organism onto the agar plate? | Wet swabs |
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